ArticleMammalian genome : official journal of the International Mammalian Genome Society1995
A linkage map with microsatellites isolated from swine flow-sorted chromosome 11.
Article in Mammalian genome : official journal of the International Mammalian Genome Society, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 16 citations in OpenAlex.
- A robust linkage map of the porcine autosomes based on gene-associated SNPs.BMC genomics · 2009Article
- The cytogenetic map of the domestic pig.Mammalian genome : official journal of the International Mammalian Genome Society · 1997Article
- Mapping of microsatellite markers developed from a flow-sorted swine chromosome 6 library.Mammalian genome : official journal of the International Mammalian Genome Society · 1997Article
- Mass production of genetic markers from a limited number of sorted chromosomes.Mammalian genome : official journal of the International Mammalian Genome Society · 1997Article
- Targeted development of microsatellite markers from the defined region of bovine chromosome 6q21-31.Mammalian genome : official journal of the International Mammalian Genome Society · 1997Article
- Construction of chromosome-specific DNA libraries covering the whole genome of field bean (Vicia faba L.).Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 1996Article
- Porcine linkage and cytogenetic maps integrated by regional mapping of 100 microsatellites on somatic cell hybrid panel.Mammalian genome : official journal of the International Mammalian Genome Society · 1996Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a simple and efficient method to construct partial libraries of swine Chromosome (Chr) 11, starting with only 300 flow-sorted copies. DNA is amplified by PARM-PCR with primer containing at the 5'-end the sequence AGCU-. After amplification, digestion of PCR products with uracil DNA glycosylase generates cohesive ends corresponding to the SstI site. The amplified fragments can then be ligated in vector linearized with the SstI enzyme. Using five different primers, we PARM-PCR amplified and cloned swine Chr 11 DNA. These chromosome-specific libraries have been used to develop 14 different (TG)n microsatellites. Ten of these markers were assigned to Chr 11 by PCR analysis of a panel of Pig-Rodent somatic hybrids and by linkage analysis of the 171 individuals of the PiGMaP reference families. A complete linkage map of 147 cM of this chromosome was then realized by integrating existing markers.
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