ArticlePlant molecular biology1995
Promoter analysis of the auxin-regulated tobacco glutathione S-transferase genes Nt103-1 and Nt103-35.
Article in Plant molecular biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Glutathione Involvement in Potato Response to French Marigold Volatile Organic Compounds.Antioxidants (Basel, Switzerland) · 2024Article
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- Plant Glutathione Transferases and Light.Frontiers in plant science · 2018Review
- Function and regulation of the glutathione peroxidase homologous gene GPXH/GPX5 in Chlamydomonas reinhardtii.Plant molecular biology · 2009Article
- Identification of NPR1-dependent and independent genes early induced by salicylic acid treatment in Arabidopsis.Plant molecular biology · 2005Article
- Salicylic acid-inducible Arabidopsis CK2-like activity phosphorylates TGA2.Plant molecular biology · 2005Article
- Functional characterization of tobacco transcription factor TGA2.1.Plant molecular biology · 2004Article
- Tobacco bZIP factor TGA10 is a novel member of the TGA family of transcription factors.Plant molecular biology · 2003Article
- The as-1 promoter element is an oxidative stress-responsive element and salicylic acid activates it via oxidative species.Plant physiology · 2002Article
- Tandemly duplicated Safener-induced glutathione S-transferase genes from Triticum tauschii contribute to genome- and organ-specific expression in hexaploid wheat.Plant physiology · 2002Article
- Analysis of the spacing between the two palindromes of activation sequence-1 with respect to binding to different TGA factors and transcriptional activation potential.Nucleic acids research · 2002Article
- A nuclear casein kinase 2 activity is involved in early events of transcriptional activation induced by salicylic acid in tobacco.Plant physiology · 2001Article
- Defence gene expression in soybean is linked to the status of the cell death program.Plant molecular biology · 2000Article
- Tobacco TGA factors differ with respect to interaction with NPR1, activation potential and DNA-binding properties.Plant molecular biology · 2000Article
- A xenobiotic-stress-activated transcription factor and its cognate target genes are preferentially expressed in root tip meristems.Plant molecular biology · 2000Article
- Selection of Arabidopsis mutants overexpressing genes driven by the promoter of an auxin-inducible glutathione S-transferase gene.Plant molecular biology · 1999Article
- DNA-binding properties, genomic organization and expression pattern of TGA6, a new member of the TGA family of bZIP transcription factors in Arabidopsis thaliana.Plant molecular biology · 1997Article
- Coordinated activation of as-1-type elements and a tobacco glutathione S-transferase gene by auxins, salicylic acid, methyl-jasmonate and hydrogen peroxide.Plant molecular biology · 1996Article
- Isolation and characterization of an auxin-inducible glutathione S-transferase gene of Arabidopsis thaliana.Plant molecular biology · 1996Article
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Authors and funding
8 authors.
Funding
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Abstract
We have analysed the promoter regions of two closely related auxin-regulated glutathione S-transferase genes. All active deletion constructs tested showed expression of the reporter gene beta-glucuronidase (gusA) in root tips of young seedlings and newly developing lateral roots. Auxin treatment greatly enhanced the level of expression. The Nt103-1 promoter region -370/-276 was found to be necessary, at least as a quantitative element to confer auxin-responsiveness to a reporter gene, and sequences responsible for the auxin-responsiveness must be located downstream of -370. The region -651/-370 contains sequence information necessary for uninduced expression. The Nt103-35 promoter manifested its auxin-responsiveness within the -504/-310 region. Electrophoretic mobility shift analysis, using nuclear extracts from tobacco leaves and suspension cells, identified a factor binding to a sequence (ap103, TGAGTCT) at position -560 of the Nt103-1 promoter, which shows homology to the mammalian AP-1 site. A second factor was found to bind a sequence (as103, ATAGCTAAGTGCTTACG) with homology to the CaMV 35S promoter as-1 element. The as103 element is present in both promoters and positioned around -360, so within the region determined to be indispensable for the response to auxin. A third factor was found binding to the -276/-190 region of both promoters. Combined, these data point to the relevance of a 90 bp region for auxin-induced activity of both tobacco genes. The ASF-1 like factor binding to the as103 element within this region might be involved in mediating the auxin response.
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