ArticleApplied and environmental microbiology1995
Xylose-metabolizing Saccharomyces cerevisiae strains overexpressing the TKL1 and TAL1 genes encoding the pentose phosphate pathway enzymes transketolase and transaldolase.
Article in Applied and environmental microbiology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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56 citing papers in PubMed, 220 citations in OpenAlex.
- Systems-Level Metabolic Regulation Strategies of Xylose Metabolism in Saccharomyces cerevisiae.Biotechnology journal · 2026Review
- In vivo pathway optimization in yeast via loxpsym-mediated shuffling of upstream activating sequences.Nucleic acids research · 2025Article
- Metabolic engineering of Saccharomyces cerevisiae for efficient utilization of pectin-rich biomass.Journal of microbiology (Seoul, Korea) · 2025Review
- Prospecting and engineering yeasts for ethanol production under inhibitory conditions: an experimental design analysis.Bioprocess and biosystems engineering · 2023Article
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- Harnessing the Endogenous 2μ Plasmid ofFrontiers in microbiology · 2021Article
- EngineeredBioengineered · 2020Review
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- Exploring the xylose paradox in Saccharomyces cerevisiae through in vivo sugar signalomics of targeted deletants.Microbial cell factories · 2019Article
- Enhanced protopanaxadiol production from xylose by engineered Yarrowia lipolytica.Microbial cell factories · 2019Article
- Pentose Metabolism in Saccharomyces cerevisiae: The Need to Engineer Global Regulatory Systems.Biotechnology journal · 2019Review
- PKA and HOG signaling contribute separable roles to anaerobic xylose fermentation in yeast engineered for biofuel production.PloS one · 2019Article
- Article
- Improvement of Lead Tolerance of Saccharomyces cerevisiae by Random Mutagenesis of Transcription Regulator SPT3.Applied biochemistry and biotechnology · 2018Article
- Enhancing the Co-utilization of Biomass-Derived Mixed Sugars by Yeasts.Frontiers in microbiology · 2018Review
- Genomic and phenotypic characterization of a refactored xylose-utilizingBiotechnology for biofuels · 2018Article
- l-Arabinose triggers its own uptake via induction of the arabinose-specific Gal2p transporter in an industrialBiotechnology for biofuels · 2018Article
- Signature pathway expression of xylose utilization in the genetically engineered industrial yeast Saccharomyces cerevisiae.PloS one · 2018Article
- Optimizing anaerobic growth rate and fermentation kinetics inBiotechnology for biofuels · 2018Article
- Quantitative metabolomics of a xylose-utilizing Saccharomyces cerevisiae strain expressing the Bacteroides thetaiotaomicron xylose isomerase on glucose and xylose.Journal of industrial microbiology & biotechnology · 2017Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Saccharomyces cerevisiae was metabolically engineered for xylose utilization. The Pichia stipitis CBS 6054 genes XYL1 and XYL2 encoding xylose reductase and xylitol dehydrogenase were cloned into S. cerevisiae. The gene products catalyze the two initial steps in xylose utilization which S. cerevisiae lacks. In order to increase the flux through the pentose phosphate pathway, the S. cerevisiae TKL1 and TAL1 genes encoding transketolase and transaldolase were overexpressed. A XYL1- and XYL2-containing S. cerevisiae strain overexpressing TAL1 (S104-TAL) showed considerably enhanced growth on xylose compared with a strain containing only XYL1 and XYL2. Overexpression of only TKL1 did not influence growth. The results indicate that the transaldolase level in S. cerevisiae is insufficient for the efficient utilization of pentose phosphate pathway metabolites. Mixtures of xylose and glucose were simultaneously consumed with the recombinant strain S104-TAL. The rate of xylose consumption was higher in the presence of glucose. Xylose was used for growth and xylitol formation, but not for ethanol production. Decreased oxygenation resulted in impaired growth and increased xylitol formation. Fermentation with strain S103-TAL, having a xylose reductase/xylitol dehydrogenase ratio of 0.5:30 compared with 4.2:5.8 for S104-TAL, did not prevent xylitol formation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.