ArticleMolecular medicine (Cambridge, Mass.)1995
Nramp transfection transfers Ity/Lsh/Bcg-related pleiotropic effects on macrophage activation: influence on oxidative burst and nitric oxide pathways.
Article in Molecular medicine (Cambridge, Mass.), 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 130 citations in OpenAlex.
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- c-Src kinase is involved in the tyrosine phosphorylation and activity of SLC11A1 in differentiating macrophages.PloS one · 2018Article
- Grandparental immune priming in the pipefish Syngnathus typhle.BMC evolutionary biology · 2017Article
- The Iron age of host-microbe interactions.EMBO reports · 2015Review
- Acute Inflammation Loci Are Involved in Wound Healing in the Mouse Ear Punch Model.Advances in wound care · 2014Review
- Salmonella acquires ferrous iron from haemophagocytic macrophages.Molecular microbiology · 2014Article
- Iron at the interface of immunity and infection.Frontiers in pharmacology · 2014Review
- Low-molecular-weight thiol-dependent antioxidant and antinitrosative defences in Salmonella pathogenesis.Molecular microbiology · 2013Article
- Slc11a1 (Nramp1) impairs growth of Salmonella enterica serovar typhimurium in macrophages via stimulation of lipocalin-2 expression.Journal of leukocyte biology · 2012Article
- Human macrophage response to L. (Viannia) panamensis: microarray evidence for an early inflammatory response.PLoS neglected tropical diseases · 2012Article
- Distinct early inflammatory events during ear tissue regeneration in mice selected for high inflammation bearing Slc11a1 R and S alleles.Inflammation · 2011Article
- Review
- Quantitative PCR-based competitive index for high-throughput screening of Salmonella virulence factors.Infection and immunity · 2011Article
- Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe Ity/Lsh/Bcg gene on mouse chromosome 1 regulates priming/activation of macrophages for antimicrobial and tumouricidal activity. A candidate gene expressed in macrophages has been identified by positional cloning and full-length sequence analysis, and encodes the Natural resistance-associated macrophage protein (Nramp). In this study, we have tested the hypothesis that the Nramp gene corresponds to Ity/Lsh/Bcg. MATERIALS AND
methodsIn vitro transfection was used to introduce the resistant allele into the macrophage cell line RAW 264.7 derived from the recessive susceptible BALB/c mouse strain. Expression of the transgene was monitored on the background of the endogenous susceptible allele by allele-specific oligonucleotide hybridization.
resultsExpression of the transgene correlated with three Lshr-associated lipopolysaccharide/interferon-gamma-regulated macrophage activation phenotypes: respiratory burst, nitrite release, and uptake of L-arginine. Endogenous and stimulated L-arginine fluxes were inhibitable with the radical scavengers nordihydroguaiaretic acid and butylated hydroxyanisole. The mitochondrial electron transport inhibitors, rotenone and thenoyltrifluoroacetone, inhibited respiratory burst, and rotenone suppressed L-arginine flux, implying that mitochondrial-derived oxygen radicals are important mediators in Nramp-regulated signal transduction pathways.
conclusionsThese data provide the first direct evidence that Nramp is the product of the Ity/Lsh/Bcg gene, and are consistent with the hypothesis that the many pleiotropic effects of this gene on macrophage activation may all derive from the requirement for mitochondrial generation of oxygen radicals for intracellular signaling.
Indexed as
Identifiers
8529105PMC2229912W263234597What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.