ArticleMolecular medicine (Cambridge, Mass.)1995
Genomic organization and sequence of the human NRAMP gene: identification and mapping of a promoter region polymorphism.
Article in Molecular medicine (Cambridge, Mass.), 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
35 citing papers in PubMed, 1 synthesis or guideline pooled it, 144 citations in OpenAlex.
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- The functional SLC11A1 gene polymorphisms are associated with sarcoidosis in Turkish population.Molecular biology reports · 2012Article
- Evidence of association with type 1 diabetes in the SLC11A1 gene region.BMC medical genetics · 2011Article
- Tuberculosis as a complex trait: impact of genetic epidemiological study design.Mammalian genome : official journal of the International Mammalian Genome Society · 2011Review
- Genetic epidemiology of tuberculosis susceptibility: impact of study design.PLoS pathogens · 2011Review
- SLC11A1 polymorphisms in inflammatory bowel disease and Mycobacterium avium subspecies paratuberculosis status.World journal of gastroenterology · 2010Article
- NRAMP1 (SLC11A1) variants: genetic susceptibility to multiple Sclerosis.Journal of clinical immunology · 2010Article
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- Linkage and association analysis of candidate genes for TB and TNFalpha cytokine expression: evidence for association with IFNGR1, IL-10, and TNF receptor 1 genes.Human genetics · 2007Article
- P2X7 and NRAMP1/SLC11 A1 gene polymorphisms in Mexican mestizo patients with pulmonary tuberculosis.Clinical and experimental immunology · 2007Article
- Relationship between Crohn's disease, infection with Mycobacterium avium subspecies paratuberculosis and SLC11A1 gene polymorphisms in Sardinian patients.World journal of gastroenterology · 2006Article
- NRAMP1 is not associated with asthma, atopy, and serum immunoglobulin E levels in the French Canadian population.Genes and immunity · 2005Article
- Susceptibility to mycobacterial infections: the importance of host genetics.Genes and immunity · 2003Review
- Mapping and sequencing of the canine NRAMP1 gene and identification of mutations in leishmaniasis-susceptible dogs.Infection and immunity · 2002Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMurine Nramp is a candidate for the macrophage resistance gene Ity/Lsh/Bcg. Sequence analysis of human NRAMP was undertaken to determine its role in man. MATERIALS AND
methodsA yeast artificial chromosome carrying NRAMP was subcloned and positive clones sequenced. The transcriptional start site was mapped using 5' RACE PCR. Polymorphic variants were amplified by PCR. Linkage analysis was used to map NRAMP.
resultsNRAMP spans 12kb and has 15 exons encoding a 550 amino acid protein showing 85% identity (92% similarity) with Nramp. Two conserved PKC sites occur in exon 2 encoding the Pro/Ser rich SH3 binding domain, and in exon 3. Striking sequence similarities (57 and 53%) were observed with yeast mitochondrial proteins, SMF1 and SMF2, especially within putative functional domains: exon 6 encoding the second transmembrane spanning domain, site of the murine susceptibility mutation; and exon 11 encoding a conserved transport motif. No mutations comparable to the murine susceptibility mutation were found. The transcriptional initiation site mapped 148 bp 5' of the translational initiation codon. 440bp of 5' flanking sequence contained putative promoter region elements: 6 interferon-gamma response elements, 3 W-elements, 3 NF kappa B binding sites and 1 AP-1 site. Nine purine-rich GGAA core motifs for the myeloid-specific PU.1 transcription factor were identified, two combining with imperfect AP1-like sites to create PEA3 motifs. TATA, GC and CCAAT boxes were absent. A possible enhancer element containing the Z-DNA forming dinucleotide repeat t(gt),ac(gt),ac(gt),g was polymorphic (4 alleles; n = 4,9,10,11), and was used to map NRAMP to 2q35.
conclusionsThis analysis provides important resources to study the role of NRAMP in human disease.
Indexed as
Identifiers
8529098PMC2229945W1483737778What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.