ArticleMolecular medicine (Cambridge, Mass.)1995
Odorant receptors and desensitization proteins colocalize in mammalian sperm.
Article in Molecular medicine (Cambridge, Mass.), 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 103 citations in OpenAlex.
- Seasonal Changes in the Seminal Plasma Proteome of the Crab-Eating Fox (Journal of proteome research · 2026Article
- In situ structure of the mouse sperm central apparatus reveals mechanistic insights into asthenozoospermia.Cell research · 2025Article
- Odorant Receptor OR2C1 Is an Essential Modulator of Boar Sperm Capacitation by Binding with Heparin.International journal of molecular sciences · 2023Article
- The influence of the female reproductive tract and sperm features on the design of microfluidic sperm-sorting devices.Journal of assisted reproduction and genetics · 2022Review
- Review
- Immunocytochemical Localization of Olfactory-signaling Molecules in Human and Rat Spermatozoa.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2020Article
- Expression of the Cerebral Olfactory Receptors Olfr110/111 and Olfr544 Is Altered During Aging and in Alzheimer's Disease-Like Mice.Molecular neurobiology · 2019Article
- System-Wide Expression and Function of Olfactory Receptors in Mammals.Genomics & informatics · 2018Review
- Odorant Receptors Containing Conserved Amino Acid Sequences in Transmembrane Domain 7 Display Distinct Expression Patterns in Mammalian Tissues.Molecules and cells · 2017Article
- A conditional Orco requirement in the somatic cyst cells for maintaining spermatids in a tight bundle in Drosophila testis.Journal of biosciences · 2016Article
- Olfactory Receptors in Non-Chemosensory Organs: The Nervous System in Health and Disease.Frontiers in aging neuroscience · 2016Review
- Impact of glycosylation on the unimpaired functions of the sperm.Clinical and experimental reproductive medicine · 2015Review
- Characterization of the Olfactory Receptors Expressed in Human Spermatozoa.Frontiers in molecular biosciences · 2015Article
- Olfactory receptors in non-chemosensory tissues.BMB reports · 2012Review
- Article
- Chemosensory Ca2+ dynamics correlate with diverse behavioral phenotypes in human sperm.The Journal of biological chemistry · 2011Article
- Rethinking the relationship between hyperactivation and chemotaxis in mammalian sperm.Biology of reproduction · 2010Review
- Expression of the G-protein alpha-subunit gustducin in mammalian spermatozoa.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2007Article
- The human olfactory receptor repertoire.Genome biology · 2001Article
- Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundThe identification of transcripts encoding putative olfactory receptors in mammalian germ cells (1) has generated the hypothesis that olfactory receptors may serve a chemosensory role in sperm chemotaxis during fertilization. We have sought to identify and localize these receptors and their regulatory machinery in rat sperm in order to gain further insight into mammalian sperm chemotaxis and odorant receptor physiology. MATERIALS AND
methodsWe conducted reverse transcription-polymerase chain reaction (RT-PCR) using degenerate primers directed against sequences conserved across members of the known odorant receptor family to identify transcripts from testis and round spermatids. Western analysis and immunohistochemistry were performed using antibodies raised against two peptide sequences conserved among odorant receptors and using fusion protein antibodies to G-protein receptor kinase 3 (GRK3/beta ARK2) and beta-arrestin2.
resultsWe detected transcripts encoding putative odorant receptors in both testis and round spermatids of the adult rat. Restriction digests of the PCR products demonstrated the existence of multiple gene products. Two anti-odorant receptor antibodies specifically recognized a 64 kD band in rat sperm preparations by Western blot. The proteins GRK3 and beta-arrestin2, implicated in olfactory desensitization, were detected in sperm cytosolic extracts using Western analysis. Immunohistochemistry colocalized putative odorant receptors, GRK3 and beta-arrestin2 to elongating spermatids in the testis and to the midpiece of mature sperm.
conclusionsThe specific localization of odorant receptors to the respiratory center of mature sperm is consistent with a role for these proteins in transducing chemotactic signals. Based on the colocalization, it is plausible that GRK3 and beta-arrestin2 function in sperm to regulate putative chemoreceptor responses.
Indexed as
Identifiers
8529092PMC2229940W2954030What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.