ArticleIn vitro cellular & developmental biology. Animal
Establishment of immortalized alveolar type II epithelial cell lines from adult rats.
Article in In vitro cellular & developmental biology. Animal. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 100 citations in OpenAlex.
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- An ErbB2 splice variant lacking exon 16 drives lung carcinoma.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- LiCoOParticle and fibre toxicology · 2020Article
- Isolation and characterisation of alveolar type II pneumocytes from adult bovine lung.Scientific reports · 2018Article
- Non-Canonical Activation of the Epidermal Growth Factor Receptor by Carbon Nanoparticles.Nanomaterials (Basel, Switzerland) · 2018Article
- Identification of an alveolar type I epithelial cell-specific DNA nuclear import sequence for gene delivery.Gene therapy · 2016Article
- Human alveolar epithelial type II cells in primary culture.Physiological reports · 2015Article
- Karyotype alteration generates the neoplastic phenotypes of SV40-infected human and rodent cells.Molecular cytogenetics · 2015Article
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- Interlaboratory evaluation of in vitro cytotoxicity and inflammatory responses to engineered nanomaterials: the NIEHS Nano GO Consortium.Environmental health perspectives · 2013Article
- Role of vitamin D-binding protein in isocyanate-induced occupational asthma.Experimental & molecular medicine · 2012Article
- Troglitazone attenuates TGF-β1-induced EMT in alveolar epithelial cells via a PPARγ-independent mechanism.PloS one · 2012Article
- A dystroglycan/plectin scaffold mediates mechanical pathway bifurcation in lung epithelial cells.The Journal of biological chemistry · 2011Article
- In vitro studies evaluating leaching of mercury from mine waste calcine using simulated human body fluids.Environmental science & technology · 2010Article
- NF-kappaB dependent and independent mechanisms of quartz-induced proinflammatory activation of lung epithelial cells.Particle and fibre toxicology · 2010Article
- Alveolar macrophage secretory products effect type 2 pneumocytes undergoing hypoxia-reoxygenation.The Annals of thoracic surgery · 2008Article
- Cytoskeletal tension regulates both expression and degradation of h2-calponin in lung alveolar cells.Biochemistry · 2006Article
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We developed methodology to isolate and culture rat alveolar Type II cells under conditions that preserved their proliferative capacity, and applied lipofection to introduce an immortalizing gene into the cells. Briefly, the alveolar Type II cells were isolated from male F344 rats using airway perfusion with a pronase solution followed by incubation for 30 min at 37 degrees C. Cells obtained by pronase digestion were predominantly epithelial in morphology and were positive for Papanicolaou and alkaline phosphatase staining. These cells could be maintained on an extracellular matrix of fibronectin and Type IV collagen in a low serum, insulin-supplemented Ham's F12 growth medium for four to five passages. Rat alveolar epithelial cells obtained by this method were transformed with the SV40-T antigen gene and two immortalized cell lines (RLE-6T and RLE-6TN) were obtained. The RLE-6T line exhibits positive nuclear immunostaining for the SV40-T antigen and the RLE-6TN line does not. PCR analysis of genomic DNA from the RLE-6T and RLE-6TN cells demonstrated the T-antigen gene was present only in the RLE-6T line indicating the RLE-6TN line is likely derived from a spontaneous transformant. After more than 50 population doublings, the RLE-6T cells stained positive for cytokeratin, possessed alkaline phosphatase activity, and contained lipid-containing inclusion bodies (phosphine 3R staining); all characteristics of alveolar Type II cells. The RLE-6TN cells exhibited similar characteristics except they did not express alkaline phosphatase activity. Early passage RLE-6T and 6TN cells showed a near diploid chromosome number.(ABSTRACT TRUNCATED AT 250 WORDS)
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