ArticleProtein science : a publication of the Protein Society1995
UME6, a negative regulator of meiosis in Saccharomyces cerevisiae, contains a C-terminal Zn2Cys6 binuclear cluster that binds the URS1 DNA sequence in a zinc-dependent manner.
Article in Protein science : a publication of the Protein Society, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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42 citing papers in PubMed, 73 citations in OpenAlex.
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- Non-redundant roles for paralogous proteins in the yeast glucose-sensing pathway.bioRxiv : the preprint server for biology · 2025Article
- Optogenetic Control of Phosphate-Responsive Genes Using Single-Component Fusion Proteins inACS synthetic biology · 2024Article
- Optogenetic control of phosphate-responsive genes using single component fusion proteins inbioRxiv : the preprint server for biology · 2024Article
- Gram-Level Production of Balanol through Regulatory Pathway and Medium Optimization in Herb FungusJournal of fungi (Basel, Switzerland) · 2022Article
- Ume6 Acts as a Stable Platform To Coordinate Repression and Activation of Early Meiosis-Specific Genes in Saccharomyces cerevisiae.Molecular and cellular biology · 2021Article
- Sharing Marks: H3K4 Methylation and H2B Ubiquitination as Features of Meiotic Recombination and Transcription.International journal of molecular sciences · 2020Review
- Systematic Study of Nucleosome-Displacing Factors in Budding Yeast.Molecular cell · 2018Article
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- Sequence-targeted nucleosome sliding in vivo by a hybrid Chd1 chromatin remodeler.Genome research · 2016Article
- Article
- Global alterations of the transcriptional landscape during yeast growth and development in the absence of Ume6-dependent chromatin modification.Molecular genetics and genomics : MGG · 2015Article
- SUB1 Plays a Negative Role during Starvation Induced Sporulation Program in Saccharomyces cerevisiae.PloS one · 2015Article
- The conserved histone deacetylase Rpd3 and its DNA binding subunit Ume6 control dynamic transcript architecture during mitotic growth and meiotic development.Nucleic acids research · 2015Article
- Single nucleotide variants in transcription factors associate more tightly with phenotype than with gene expression.PLoS genetics · 2014Article
- Acetylation of the transcriptional repressor Ume6p allows efficient promoter release and timely induction of the meiotic transient transcription program in yeast.Molecular and cellular biology · 2014Article
- The roles of the catalytic and noncatalytic activities of Rpd3L and Rpd3S in the regulation of gene transcription in yeast.PloS one · 2013Article
- The linker histone plays a dual role during gametogenesis in Saccharomyces cerevisiae.Molecular and cellular biology · 2012Article
- Ume6 is required for the MATa/MATalpha cellular identity and transcriptional silencing in Kluyveromyces lactis.Genetics · 2010Article
- Genome reprogramming during sporulation.The International journal of developmental biology · 2009Review
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4 authors at 2 institutions in 1 country.
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Abstract
UME6 is a protein of 836 amino acids from Saccharomyces cerevisiae that acts as a repressor and activator of several early meiotic genes. UME6 contains, near the C-terminus, the amino acid sequence-771C-X2-C-X6-C-X6-C-X2-C-X6-C-, in which the spacings of the six Cys residues are identical to those found in 39 N-terminal Cys-rich DNA binding subdomains of fungal transcription factors. This sequence has been shown in GAL4 and other proteins to form a zinc binuclear cluster. In spite of the different location, the C-rich sequence, cloned and over-produced within the last 111 amino acid residues of UME6, UME6(111), forms a binuclear cluster and exhibits a Zn-dependent binding to the URS1 DNA sequence. The latter, TAGCCGCCGA, is required for the repression or activation of meiosis-specific genes by UME6. UME6(111) contains 1.8 +/- 0.4 mol Zn/mol protein and the Zn can be exchanged for Cd to yield a protein containing 1.9 +/- 0.1 mol Cd/mol protein. At 5 degrees C, 113Cd2UME6(111) shows two 113Cd NMR signals, with chemical shifts of 699 and 689 ppm, similar to those observed for 113Cd2GAL4(149). The magnitude of these chemical shifts suggests that each 113Cd nucleus is coordinated to four -S- ligands, compatible with a 113Cd2 cluster structure in which two thiolates from bridging ligands. The entire UME6 gene has been cloned and overexpressed and binds more tightly to the URS1 sequence than the zinc binuclear cluster domain alone. DNase I footprints of UME6 on URS1-containing DNA show that the protein protects the phosphodiesters of the 5'-CCGCCG-3' region within the URS1 sequence.
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