ArticleBiophysical journal1995
Fluorescence of membrane-bound tryptophan octyl ester: a model for studying intrinsic fluorescence of protein-membrane interactions.
Article in Biophysical journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 72 citations in OpenAlex.
- Sited-Directed Fluorescence Spectroscopy for Studies on the Folding and Insertion of Proteins in Lipid Bilayers and Model Membranes.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Fluorescent Probes and Quenchers in Studies of Protein Folding and Protein-Lipid Interactions.Chemical record (New York, N.Y.) · 2024Review
- Determination of the boundary lipids of sticholysins using tryptophan quenching.Scientific reports · 2022Article
- Measuring Membrane Penetration Depths and Conformational Changes in Membrane Peptides and Proteins.The Journal of membrane biology · 2022Review
- Transient Excursions to Membrane Core as Determinants of Influenza Virus Fusion Peptide Activity.International journal of molecular sciences · 2021Article
- The Secret Lives of Fluorescent Membrane Probes as Revealed by Molecular Dynamics Simulations.Molecules (Basel, Switzerland) · 2020Review
- Location of TEMPO-PC in Lipid Bilayers: Implications for Fluorescence Quenching.The Journal of membrane biology · 2020Article
- Measuring membrane penetration with depth-dependent fluorescence quenching: distribution analysis is coming of age.Biochimica et biophysica acta · 2014Review
- Theoretical study of the NLO responses of some natural and unnatural amino acids used as probe molecules.Journal of molecular modeling · 2014Article
- Refining membrane penetration by a combination of steady-state and time-resolved depth-dependent fluorescence quenching.Analytical biochemistry · 2014Article
- Validation of depth-dependent fluorescence quenching in membranes by molecular dynamics simulation of tryptophan octyl ester in POPC bilayer.The journal of physical chemistry. B · 2013Article
- Spectroscopic and computational study of melittin, cecropin A, and the hybrid peptide CM15.The journal of physical chemistry. B · 2012Article
- Characterization of perfluorooctylbromide-based nanoemulsion particles using atomistic molecular dynamics simulations.The journal of physical chemistry. B · 2010Article
- Fluorescence and UV resonance Raman study of peptide-vesicle interactions of human cathelicidin LL-37 and its F6W and F17W mutants.Biochemistry · 2009Article
- Hydrogen bonding and solvent polarity markers in the uv resonance raman spectrum of tryptophan: application to membrane proteins.The journal of physical chemistry. B · 2009Article
- Dynamics of a membrane-bound tryptophan analog in environments of varying hydration: a fluorescence approach.European biophysics journal : EBJ · 2005Article
- Nanosecond dynamics of a mimicked membrane-water interface observed by time-resolved stokes shift of LAURDAN.Biophysical journal · 2005Article
- Interaction of melittin with membrane cholesterol: a fluorescence approach.Biophysical journal · 2004Article
- Tethered polymer-supported planar lipid bilayers for reconstitution of integral membrane proteins: silane-polyethyleneglycol-lipid as a cushion and covalent linker.Biophysical journal · 2000Article
- Tryptophan octyl ester in detergent micelles of dodecylmaltoside: fluorescence properties and quenching by brominated detergent analogs.Biophysical journal · 1999Article
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2 authors at 1 institution in 1 country.
Funding
Abstract
The fluorescence of a membrane-bound tryptophan derivative (tryptophan octyl ester, TOE) has been examined as a model for tryptophan fluorescence from proteins in membrane environments. The depth-dependent fluorescence quenching of TOE by brominated lipids was found to proceed via a dynamic mechanism with vertical fluctuations playing a central role in the process. The activation energy for the quenching was estimated to be 1.3 kcal/mole. The data were analyzed using the distribution analysis (DA) method, which extends the conventional parallax method to account more realistically for the transbilayer distributions of both probe and quencher and for possible variations in the probe's accessibility. DA provides a better fit than the parallax method to data collected with TOE in membranes formed of lipids brominated at either the 4,5, the 6,7, the 9,10, or the 11,12 positions of the sn-2 acyl chain. DA yields information on the fluorophore's most probable depth in the membrane, its conformational heterogeneity, and its accessibility to the lipid phase. Previously reported data on cytochrome b5 and melittin were reanalyzed together with data obtained with TOE. This new analysis demonstrates conformational heterogeneity in melittin and provides estimates of the freedom of motion and exposure to the lipid phase of membrane-embedded tryptophans of cytochrome b5.
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