ArticleBiophysical journal1995
Measurement of erythrocyte membrane elasticity by flicker eigenmode decomposition.
Article in Biophysical journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed, 204 citations in OpenAlex.
- Stomatocyte-discocyte-echinocyte transformations of erythrocyte modulated by membrane-cytoskeleton mechanical properties.Biophysical journal · 2025Article
- Active membrane deformations of a minimal synthetic cell.Nature physics · 2025Article
- Red blood cell flickering activity locally controlled by holographic optical tweezers.iScience · 2024Article
- MEDUSA: A cloud-based tool for the analysis of X-ray diffuse scattering to obtain the bending modulus from oriented membrane stacks.PLoS computational biology · 2024Article
- Modulation of a rapid neurotransmitter receptor-ion channel by membrane lipids.Frontiers in cell and developmental biology · 2023Review
- Remodeling of the Plasma Membrane by Surface-Bound Protein Monomers and Oligomers: The Critical Role of Intrinsically Disordered Regions.The Journal of membrane biology · 2022Review
- Morphological Characteristics, Hemoglobin Content, and Membrane Mechanical Properties of Red Blood Cell Delivery Systems.ACS applied materials & interfaces · 2022Article
- A flexible Bayesian framework for unbiased estimation of timescales.Nature computational science · 2022Article
- Structural and mechanical properties of the red blood cell's cytoplasmic membrane seen through the lens of biophysics.Frontiers in physiology · 2022Review
- Article
- On the Role of Curved Membrane Nanodomains, and Passive and Active Skeleton Forces in the Determination of Cell Shape and Membrane Budding.International journal of molecular sciences · 2021Article
- Doppler imaging detects bacterial infection of living tissue.Communications biology · 2021Article
- Differential dielectroscopic data on the relation of erythrocyte membrane skeleton to erythrocyte deformability and flicker.European biophysics journal : EBJ · 2021Article
- Constitutive Model of Erythrocyte Membranes with Distributions of Spectrin Orientations and Lengths.Biophysical journal · 2020Article
- Histological and SEM Assessment of Blood Stasis in Kidney Blood Vessels after Repeated Intra-Arterial Application of Radiographic Contrast Media.Life (Basel, Switzerland) · 2020Article
- Heat damage of cytoskeleton in erythrocytes increases membrane roughness and cell rigidity.Journal of biological physics · 2019Article
- Optical Tracking of Nanometer-Scale Cellular Membrane Deformation Associated with Single Vesicle Release.ACS sensors · 2019Article
- Doppler fluctuation spectroscopy of intracellular dynamics in living tissue.Journal of the Optical Society of America. A, Optics, image science, and vision · 2019Article
- Quantification of stored red blood cell fluctuations by time-lapse holographic cell imaging.Biomedical optics express · 2018Article
- Imaging Action Potential in Single Mammalian Neurons by Tracking the Accompanying Sub-Nanometer Mechanical Motion.ACS nano · 2018Article
2 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have studied the flickering of erythrocytes at wavelengths comparable to the cell dimension. To do this we have analyzed the edge fluctuations of the cell to a resolution of 5 nm by combining phase contrast microscopy with fast image processing. By measuring the edge excitations simultaneously at four orthogonal positions around the cell, the eigenmodes of equal azimuthal mode numbers m = 0,1,2 could be separated. From a continuous time sequence of 100 s of video frames taken at 40 ms time intervals, we determined the time-auto correlation function for the modes m = 0,1,2 and calculated their mean square amplitudes <delta n2m> as well as their decay times tau m. To explain the results we also present the theoretically calculated energy eigenmodes of an erythrocyte, accounting for the constraint that the cell is in contact with the substrate along an annular ring, which agreed well with the experimental findings. We found that the softest mode is a "hindered translational" mode with m = 1 of the adhered cell, which is almost insensitive to the shear elastic modulus. Comparison of the calculated and measured amplitudes yielded an average value for the bending stiffness of kc = 4 x 10(-19) J, which is much larger than the value obtained by flicker analysis at short wavelengths (kc = 2.3 x 10(-20) J). It would, however, agree well with the value expected from the red cell membrane area compressibility modulus of K = 4.5 x 10(-1)N/m, which corresponds to a lipid bilayer containing approximately 50 mol % of cholesterol. In contradiction to our theoretical expectations we found that the flicker eigenmodes seemed not to be influenced by the membrane shear elasticity, which will be discussed in terms of an unusual coupling between the lipid bilayer and the cytoskeleton.
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