ArticleBiophysical journal1995
Hydrophobic ions amplify the capacitive currents used to measure exocytotic fusion.
Article in Biophysical journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 26 citations in OpenAlex.
- Plasma membrane-endoplasmic reticulum coupling probed with genetically-encoded voltage sensors.bioRxiv : the preprint server for biology · 2025Article
- Optical probing of local membrane potential with fluorescent polystyrene beads.Biophysical reports · 2021Article
- Imaging the electrical activity of organelles in living cells.Communications biology · 2021Article
- A dark quencher genetically encodable voltage indicator (dqGEVI) exhibits high fidelity and speed.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- High-resolution membrane capacitance measurements for the study of exocytosis and endocytosis.Nature protocols · 2013Article
- Mechanistic analysis of massive endocytosis in relation to functionally defined surface membrane domains.The Journal of general physiology · 2011Article
- Improved probes for hybrid voltage sensor imaging.Biophysical journal · 2010Article
- Submillisecond optical reporting of membrane potential in situ using a neuronal tracer dye.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Effect of voltage sensitive fluorescent proteins on neuronal excitability.Biophysical journal · 2009Article
- Effects on membrane capacitance of steroids with antagonist properties at GABAA receptors.Biophysical journal · 2008Article
- A combined patch-clamp and electrorotation study of the voltage- and frequency-dependent membrane capacitance caused by structurally dissimilar lipophilic anions.The Journal of membrane biology · 2008Article
- Voltage-dependent dynamic FRET signals from the transverse tubules in mammalian skeletal muscle fibers.The Journal of general physiology · 2007Article
- Secretory vesicles membrane area is regulated in tandem with quantal size in chromaffin cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
- Trafficking, assembly, and function of a connexin43-green fluorescent protein chimera in live mammalian cells.Molecular biology of the cell · 1999Article
- Modulation of the unitary exocytic event amplitude by cAMP in rat melanotrophs.The Journal of physiology · 1998Article
- Interaction of lipophilic ions with the plasma membrane of mammalian cells studies by electrorotation.Biophysical journal · 1998Article
- Regulation of exocytotic fusion by cell inflation.Biophysical journal · 1998Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The detection of exocytotic fusion in patch-clamped secretory cells depends on measuring an increase in the cell membrane capacitance as new membrane is added to the plasma membrane. However, in the majority of secretory cells, secretory vesicles are too small (< 200 nm in diameter) to cause a detectable signal. We have found that incubations of normal mouse mast cells with the hydrophobic anion dipicrylamine (DPA), increases cell membrane capacitance by about three times. The large capacitive current induced by DPA was voltage-dependent, having a maximum value at -10 mV. The DPA-induced charge movement could be described by a single barrier model in which the DPA molecules move between two stable states in the bulk lipid matrix of the membrane. More importantly, the DPA treatment produced a sevenfold increase in the size of the capacitance steps observed upon the exocytotic fusion of single secretory granules. A similar amplification of DPA on the secretory vesicle capacitance was observed in a cell with larger (< or = 5 microns in diameter) or with smaller secretory granules (< 250 nm in diameter). Additionally, the increased granule membrane capacitance enlarged the transient capacitive discharge measured upon formation of a fusion pore in normal mast cell granules. Our results indicate that hydrophobic ions provide an important tool for high resolution studies of membrane capacitance.
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