ArticleBiophysical journal1995
Real time imaging of single fluorophores on moving actin with an epifluorescence microscope.
Article in Biophysical journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Single-Nanoparticle Tracking with Angstrom Localization Precision and Microsecond Time Resolution.Biophysical journal · 2018Article
- Single-molecule fluorescence-based analysis of protein conformation, interaction, and oligomerization in cellular systems.Biophysical reviews · 2018Review
- How we discovered fluorescent speckle microscopy.Molecular biology of the cell · 2011Article
- Single-molecule spectroscopy and imaging of biomolecules in living cells.Analytical chemistry · 2010Article
- Homebuilt single-molecule scanning confocal fluorescence microscope studies of single DNA/protein interactions.Methods (San Diego, Calif.) · 2007Article
- Measurement of single macromolecule orientation by total internal reflection fluorescence polarization microscopy.Biophysical journal · 2005Article
- A new muscle contractile system composed of a thick filament lattice and a single actin filament.Biophysical journal · 2005Article
- Manipulation of a Large DNA Molecule using the Phase Transition.Journal of biological physics · 2002Article
- Single molecule imaging of green fluorescent proteins in living cells: E-cadherin forms oligomers on the free cell surface.Biophysical journal · 2001Article
- Characterization of single actomyosin rigor bonds: load dependence of lifetime and mechanical properties.Biophysical journal · 2000Article
- Stepping rotation of F1-ATPase visualized through angle-resolved single-fluorophore imaging.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Imaging and tracking of single GFP molecules in solution.Biophysical journal · 2000Article
- Properties of lipid microdomains in a muscle cell membrane visualized by single molecule microscopy.The EMBO journal · 2000Article
- Free Brownian motion of individual lipid molecules in biomembranes.Biophysical journal · 1999Article
- Imaging of thermal activation of actomyosin motors.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Single-molecule fluorescence spectroscopy of enzyme conformational dynamics and cleavage mechanism.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- How microtubules get fluorescent speckles.Biophysical journal · 1998Article
- Detection of dimers of dimers of human leukocyte antigen (HLA)-DR on the surface of living cells by single-particle fluorescence imaging.The Journal of cell biology · 1998Article
- Single-molecule microscopy on model membranes reveals anomalous diffusion.Biophysical journal · 1997Article
- Nonexponential kinetics of a single tRNAPhe molecule under physiological conditions.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
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Authors and funding
5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Relatively simple modifications of an ordinary epifluorescence microscope have greatly reduced its background luminescence, allowing continuous and real time imaging of single fluorophores in an aqueous medium. Main modifications were changing the excitation light path and setting an aperture stop so that stray light does not scatter inside the microscope. A simple and accurate method using actin filaments is presented to establish the singularity of the observed fluorophores. It was possible, at the video rate of 30 frames/s, to image individual tetramethylrhodamine fluorophores bound to actin filaments sliding over heavy meromyosin. The successful imaging of moving fluorophores demonstrates that conventional microscopes may become a routine tool for studying dynamic interactions among individual biomolecules in physiological environments.
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