ArticleApplied and environmental microbiology1995
Genotyping male-specific RNA coliphages by hybridization with oligonucleotide probes.
Article in Applied and environmental microbiology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
38 citing papers in PubMed, 178 citations in OpenAlex.
- Searching for a Reliable Viral Indicator of Faecal Pollution in Aquatic Environments.Journal of microbiology (Seoul, Korea) · 2023Review
- Bacteriophages in sewage: abundance, roles, and applications.FEMS microbes · 2022Article
- Bacteriophages as Fecal Pollution Indicators.Viruses · 2021Review
- Male-Specific and Somatic Coliphage Profiles from Major Aquaculture Areas in Republic of Korea.Food and environmental virology · 2020Article
- Overview of Monitoring Techniques for Evaluating Water Quality at Potable Reuse Treatment Facilities.Journal - American Water Works Association · 2019Article
- Occurrence of microbial indicators, pathogenic bacteria and viruses in tropical surface waters subject to contrasting land use.Water research · 2019Article
- Toward Forensic Uses of Microbial Source Tracking.Microbiology spectrum · 2018Article
- Bacteriophages as indicators of faecal pollution and enteric virus removal.Letters in applied microbiology · 2017Review
- Preliminary Source Tracking of Male-Specific (FFood and environmental virology · 2017Article
- Legacy of contaminant N sources to the NOScientific reports · 2017Article
- Waterborne Viruses and F-Specific Coliphages in Mixed-Use Watersheds: Microbial Associations, Host Specificities, and Affinities with Environmental/Land Use Factors.Applied and environmental microbiology · 2017Article
- Quantitative Distribution of Infectious F-Specific RNA Phage Genotypes in Surface Waters.Applied and environmental microbiology · 2016Article
- The Interaction between Heterotrophic Bacteria and Coliform, Fecal Coliform, Fecal Streptococci Bacteria in the Water Supply Networks.Iranian journal of public health · 2015Article
- Application of F⁺RNA Coliphages as Source Tracking Enteric Viruses on Parsley and Leek Using RT-PCR.Food and environmental virology · 2015Article
- Enzyme treatment reverse transcription-PCR to differentiate infectious and inactivated F-specific RNA phages.Applied and environmental microbiology · 2014Article
- Microbial monitoring of surface water in South Africa: an overview.International journal of environmental research and public health · 2012Review
- Occurrence, survival, and persistence of human adenoviruses and F-specific RNA phages in raw groundwater.Applied and environmental microbiology · 2010Article
- Viral multiplex quantitative PCR assays for tracking sources of fecal contamination.Applied and environmental microbiology · 2010Article
- Molecular characterization of bacteriophages for microbial source tracking in Korea.Applied and environmental microbiology · 2009Article
- Evaluation of two library-independent microbial source tracking methods to identify sources of fecal contamination in French estuaries.Applied and environmental microbiology · 2007Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
F-specific (F+) RNA coliphages are prevalent in sewage and other fecal wastes of humans and animals. There are four antigenically distinct serogroups of F+ RNA coliphages, and those predominating in humans (groups II and III) differ from those predominating in animals (groups I and IV). Hence, it may be possible to distinguish between human and animal wastes by serotyping F+ RNA coliphage isolates. Because serotyping is laborious and requires scarce antiserum reagents, we investigated genotyping using synthetic oligonucleotide probes as an alternative approach to distinguishing the four groups of F+ RNA coliphages. Oligoprobes I, II, III, IV, A, and B were selected to detect group I, II, III, IV, I plus II, and III plus IV phages, respectively. Methods for phage transfer from zones of lysis on a host cell lawn to candidate membrane filters and fixation of genomic nucleic acid on the membranes were optimized. The oligoprobes, which were end labeled with digoxigenin, were applied in DNA-RNA hybridization, and hybrids were observed by colorimetric, immunoenzymatic detection. Of 203 isolates of F+ RNA coliphages from environmental samples of water, wastes, and shellfish, 99.5 and 96.6% could be classified into each group by serotyping and genotyping, respectively. Probes A and B correctly identified 100% of the isolates. On the basis of these results, this method for genotyping F+ RNA coliphages appears to be practical and reliable for typing isolates in field samples.
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