ArticleProceedings of the National Academy of Sciences of the United States of America1995
Expression studies of catalytic antibodies.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 82 citations in OpenAlex.
- Pioneer: a synthetic human antibody phage display library for rapid therapeutic lead generation.mAbs · 2025Article
- Adaptive mutations alter antibody structure and dynamics during affinity maturation.Biochemistry · 2015Article
- Protein dynamics and the diversity of an antibody response.The Journal of biological chemistry · 2012Article
- Site-specific coupling and sterically controlled formation of multimeric antibody fab fragments with unnatural amino acids.Journal of molecular biology · 2011Article
- An aspartate and a water molecule mediate efficient acid-base catalysis in a tailored antibody pocket.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Strategies for successful recombinant expression of disulfide bond-dependent proteins in Escherichia coli.Microbial cell factories · 2009Article
- Single-chain Fv phage display propensity exhibits strong positive correlation with overall expression levels.BMC biotechnology · 2008Article
- Antibody evolution constrains conformational heterogeneity by tailoring protein dynamics.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Protein dynamics and the immunological evolution of molecular recognition.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Molecular mechanism of enantioselective proton transfer to carbon in catalytic antibody 14D9.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Structural evidence for substrate strain in antibody catalysis.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Flexibility and molecular recognition in the immune system.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- A phage display approach for rapid antibody humanization: designed combinatorial V gene libraries.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Phage display of a catalytic antibody to optimize affinity for transition-state analog binding.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Insights into antibody catalysis: structure of an oxygenation catalyst at 1.9-angstrom resolution.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
We have examined the positive influence of human constant regions on the folding and bacterial expression of active soluble mouse immunoglobulin variable domains derived from a number of catalytic antibodies. Expression yields of eight hybridoma- and myeloma-derived chimeric Fab fragments are compared in both shake flasks and high density fermentations. In addition the usefulness of this system for the generation of in vivo expression libraries is examined by constructing and expressing combinations of heavy and light chain variable regions that were not selected as a pair during an immune response. A mutagenesis study of one of the recombinant catalytic Fab fragments reveals that single amino acid substitutions can have dramatic effects on the expression yield. This system should be generally applicable to the production of Fab fragments of catalytic and other hybridoma-derived antibodies for crystallographic and structure-function studies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.