ArticleProceedings of the National Academy of Sciences of the United States of America1995
Projection structure of frog rhodopsin in two crystal forms.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 217 citations in OpenAlex.
- Rhodopsins: An Excitingly Versatile Protein Species for Research, Development and Creative Engineering.Frontiers in chemistry · 2022Review
- Supramolecular organization of rhodopsin in rod photoreceptor cell membranes.Pflugers Archiv : European journal of physiology · 2021Review
- Conformational perturbation, allosteric modulation of cellular signaling pathways, and disease in P23H rhodopsin.Scientific reports · 2020Article
- Unconventional Roles of Opsins.Annual review of cell and developmental biology · 2017Review
- Disruption of Rhodopsin Dimerization with Synthetic Peptides Targeting an Interaction Interface.The Journal of biological chemistry · 2015Article
- Chemistry and biology of the initial steps in vision: the Friedenwald lecture.Investigative ophthalmology & visual science · 2014Article
- Preferential binding of an odor within olfactory receptors: a precursor to receptor activation.Chemical senses · 2014Article
- Computational approaches for modeling GPCR dimerization.Current pharmaceutical biotechnology · 2014Review
- Beyond standard molecular dynamics: investigating the molecular mechanisms of G protein-coupled receptors with enhanced molecular dynamics methods.Advances in experimental medicine and biology · 2014Review
- GPCR: G protein complexes--the fundamental signaling assembly.Amino acids · 2013Review
- The rat adenine receptor: pharmacological characterization and mutagenesis studies to investigate its putative ligand binding site.Purinergic signalling · 2013Article
- Modeling of mammalian olfactory receptors and docking of odorants.Biophysical reviews · 2012Review
- Structural determinants of the supramolecular organization of G protein-coupled receptors in bilayers.Journal of the American Chemical Society · 2012Article
- Article
- The impact of GPCR structures on pharmacology and structure-based drug design.British journal of pharmacology · 2010Review
- Phospholipids are needed for the proper formation, stability, and function of the photoactivated rhodopsin-transducin complex.Biochemistry · 2009Article
- Rhodopsin and the others: a historical perspective on structural studies of G protein-coupled receptors.Current pharmaceutical design · 2009Review
- Modeling dimerizations of transmembrane proteins using Brownian dynamics simulations.Journal of computer-aided molecular design · 2008Article
- Advances in the Development and Application of Computational Methodologies for Structural Modeling of G-Protein Coupled Receptors.Expert opinion on drug discovery · 2008Article
- A strategy using NMR peptide structures of thromboxane A2 receptor as templates to construct ligand-recognition pocket of prostacyclin receptor.BMC biochemistry · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Rhodopsin is the G protein-coupled receptor that upon light activation triggers the visual transduction cascade. Rod cell outer segment disc membranes were isolated from dark-adapted frog retinas and were extracted with Tween detergents to obtain two-dimensional rhodopsin crystals for electron crystallography. When Tween 80 was used, tubular structures with a p2 lattice (a = 32 A, b = 83 A, gamma = 91 degrees) were formed. The use of a Tween 80/Tween 20 mixture favored the formation of larger p22(1)2(1) lattices (a = 40 A, b = 146 A, gamma = 90 degrees). Micrographs from frozen hydrated frog rhodopsin crystals were processed, and projection structures to 7-A resolution for the p22(1)2(1) form and to 6-A resolution for the p2 form were calculated. The maps of frog rhodopsin in both crystal forms are very similar to the 9-A map obtained previously for bovine rhodopsin and show that the arrangement of the helices is the same. In a tentative topographic model, helices 4, 6, and 7 are nearly perpendicular to the plane of the membrane. In the higher-resolution projection maps of frog rhodopsin, helix 5 looks more tilted than it appeared previously. The quality of the two frog rhodopsin crystals suggests that they would be suitable to obtain a three-dimensional structure in which all helices would be resolved.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.