ArticleNucleic acids research1995
The ribosomal neighbourhood of the central fold of tRNA: cross-links from position 47 of tRNA located at the A, P or E site.
Article in Nucleic acids research, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 48 citations in OpenAlex.
- Time-Dependent Analysis ofACS omega · 2021Article
- Mitochondrial ribosome assembly in health and disease.Cell cycle (Georgetown, Tex.) · 2015Review
- The DEAD box protein Mrh4 functions in the assembly of the mitochondrial large ribosomal subunit.Cell metabolism · 2013Article
- rRNA mutations that inhibit transfer-messenger RNA activity on stalled ribosomes.Journal of bacteriology · 2010Article
- Ribosome performance is enhanced by a rich cluster of pseudouridines in the A-site finger region of the large subunit.The Journal of biological chemistry · 2008Article
- The process of mRNA-tRNA translocation.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- 5S rRNA: Structure and Function from Head to Toe.International journal of biomedical science : IJBS · 2005Article
- 5S rRNA: Structure and Function from Head to Toe.International journal of biomedical science : IJBS · 2005Article
- Crystal structure of ribosomal protein L27 from Thermus thermophilus HB8.Protein science : a publication of the Protein Society · 2004Article
- Orientation of the tRNA anticodon in the ribosomal P-site: quantitative footprinting with U33-modified, anticodon stem and loop domains.RNA (New York, N.Y.) · 1999Article
- Neighborhood of 16S rRNA nucleotides U788/U789 in the 30S ribosomal subunit determined by site-directed crosslinking.RNA (New York, N.Y.) · 1998Article
- Survey of four different photoreactive moieties for DNA photoaffinity labeling of yeast RNA polymerase III transcription complexes.Nucleic acids research · 1998Article
- A novel ribosome-associated protein is important for efficient translation in Escherichia coli.Journal of bacteriology · 1997Article
- Synthesis and evaluation of oligodeoxyribonucleotides containing an aryl(trifluoromethyl)diazirine moiety as the cross-linking probe: photoaffinity labeling of mammalian DNA polymerase beta.Nucleic acids research · 1997Article
- Transfer RNA docking pair model in the ribosomal pre- and post-translocational states.Nucleic acids research · 1997Article
- Loop IV of 5S ribosomal RNA has contacts both to domain II and to domain V of the 23S RNA.RNA (New York, N.Y.) · 1996Article
- A base substitution in the amino acid acceptor stem of tRNA(Lys) causes both misacylation and altered decoding.Gene expression · 1996Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The naturally occurring nucleotide 3-(3-amino-3-carboxy-propyl)uridine (acp3U) at position 47 of tRNA(Phe) from Escherichia coli was modified with a diazirine derivative and bound to ribosomes in the presence of suitable mRNA analogues under conditions specific for the ribosomal A, P or E sites. After photo-activation at 350 nm the cross-links to ribosomal proteins and RNA were identified by our standard procedures. In the 30S subunit protein S19 (and weakly S9 and S13) was the target of cross-linking from tRNA at the A site, S7, S9 and S13 from the P site and S7 from the E site. Similarly, in the 50S subunit L16 and L27 were cross-linked from the A site, L1, L5, L16, L27 and L33 from the P site and L1 and L33 from the E site. Corresponding cross-links to rRNA were localized by RNase H digestion to the following areas: in 16S rRNA between positions 687 and 727 from the P and E sites, positions 1318 and 1350 (P site) and 1350 and 1387 (E site); in the 23S rRNA between positions 865 and 910 from the A site, 1845 and 1892 (P site), 1892 and 1945 (A site), 2282 and 2358 (P site), 2242 and 2461 (P and E sites), 2461 and 2488 (A site), 2488 and 2539 (all three sites) and 2572 and 2603 (A and P sites). In most (but not all) cases, more precise localizations of the cross-link sites could be made by primer extension analysis.
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