ArticleNucleic acids research1995
Cysteine tRNAs of plant origin as novel UGA suppressors.
Article in Nucleic acids research, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed.
- Comprehensive analysis of chloroplast genome evolution in Poaceae: codon usage patterns, selection pressures, and phylogenomic relationships.BMC genomics · 2026Article
- Factors Affecting Readthrough of Natural Versus Premature Termination Codons.Advances in experimental medicine and biology · 2023Review
- Tissue-specific regulation of translational readthrough tunes functions of the traffic jam transcription factor.Nucleic acids research · 2022Article
- Overview of tRNA Modifications in Chloroplasts.Microorganisms · 2022Article
- Review
- The functional readthrough extension of malate dehydrogenase reveals a modification of the genetic code.Open biology · 2016Article
- Nonsense suppression by near-cognate tRNAs employs alternative base pairing at codon positions 1 and 3.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Translational readthrough potential of natural termination codons in eucaryotes--The impact of RNA sequence.RNA biology · 2015Review
- New insights into the incorporation of natural suppressor tRNAs at stop codons in Saccharomyces cerevisiae.Nucleic acids research · 2014Article
- In vitro incorporation of nonnatural amino acids into protein using tRNA(Cys)-derived opal, ochre, and amber suppressor tRNAs.RNA (New York, N.Y.) · 2010Article
- Penultimate selenocysteine residue replaced by cysteine in thioredoxin reductase from selenium-deficient rat liver.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2009Article
- Control of gag-pol gene expression in the Candida albicans retrotransposon Tca2.BMC molecular biology · 2007Article
- A comparative genomics analysis of codon reassignments reveals a link with mitochondrial proteome size and a mechanism of genetic code change via suppressor tRNAs.Journal of molecular evolution · 2007Article
- Identification of a tRNA isopentenyltransferase gene from Arabidopsis thaliana.Plant molecular biology · 2002Article
- Misreading of termination codons in eukaryotes by natural nonsense suppressor tRNAs.Nucleic acids research · 2001Review
- Nucleotide sequences and functional characterization of two tobacco UAG suppressor tRNA(Gln) isoacceptors and their genes.Plant molecular biology · 1998Article
- Wheat cytoplasmic arginine tRNA isoacceptor with a U*CG anticodon is an efficient UGA suppressor in vitro.Nucleic acids research · 1998Article
- Nucleotide sequences of nuclear tRNA(Cys) genes from Nicotiana and Arabidopsis and expression in HeLa cell extract.Plant molecular biology · 1996Article
- Gene expression from viral RNA genomes.Plant molecular biology · 1996Review
- UGA suppression by tRNACmCATrp occurs in diverse virus RNAs due to a limited influence of the codon context.Nucleic acids research · 1996Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have isolated and sequenced chloroplast (chl) and cytoplasmic (cyt) cysteine tRNAs from Nicotiana rustica. Both tRNAs carry a GCA anticodon but beyond that differ considerably in their nucleotide sequences. One obvious distinction resides in the presence of N6-isopentenyladenosine (i6A) and 1-methylguanosine (m1G) at position 37 in chl and cyt tRNA(Cys) respectively. In order to study the potential suppressor activity of tRNAs(Cys) we used in vitro synthesized zein mRNA transcripts in which an internal UGA stop codon had been placed in either the tobacco rattle virus (TRV)- or tobacco mosaic virus (TMV)-specific codon context. In vitro translation was carried out in a messenger- and tRNA-dependent wheat germ extract. Both tRNA(Cys) isoacceptors stimulate read-through over the UGA stop codon, however, chl tRNA(GCA)Cys is more efficient than the cytoplasmic counterpart. The UGA in the two viral codon contexts is suppressed to about the same extent by either of the two tRNAs(Cys), whereas UGA in the beta-globin context is not recognized at all. The interaction of tRNA(GCA)Cys with UGA requires an unconventional G:A base pair in the wobble position, as postulated earlier for plant tRNA(G psi A)Tyr misreading the UAA stop codon. This is the first case that a cysteine-accepting tRNA has been characterized as a natural UGA suppressor.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.