ArticleMolecular and cellular biology1996
Stockpiling of Cdc25 during a DNA replication checkpoint arrest in Schizosaccharomyces pombe.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 71 citations in OpenAlex.
- Uncoupling of Mitosis and Cytokinesis Upon a Prolonged Arrest in Metaphase Is Influenced by Protein Phosphatases and Mitotic Transcription in Fission Yeast.Frontiers in cell and developmental biology · 2022Article
- Review
- Caffeine as a tool for investigating the integration of Cdc25 phosphorylation, activity and ubiquitin-dependent degradation inCell division · 2020Review
- Pharmacophore-guided discovery of CDC25 inhibitors causing cell cycle arrest and tumor regression.Scientific reports · 2019Article
- Size-Dependent Expression of the Mitotic Activator Cdc25 Suggests a Mechanism of Size Control in Fission Yeast.Current biology : CB · 2017Article
- Wee1 and Cdc25 are controlled by conserved PP2A-dependent mechanisms in fission yeast.Cell cycle (Georgetown, Tex.) · 2017Article
- The centrosome and its duplication cycle.Cold Spring Harbor perspectives in biology · 2015Review
- Hyperactive Cdc2 kinase interferes with the response to broken replication forks by trapping S.pombe Crb2 in its mitotic T215 phosphorylated state.Nucleic acids research · 2014Article
- Caffeine stabilizes Cdc25 independently of Rad3 in Schizosaccharomyces pombe contributing to checkpoint override.Molecular microbiology · 2014Article
- Torin1-mediated TOR kinase inhibition reduces Wee1 levels and advances mitotic commitment in fission yeast and HeLa cells.Journal of cell science · 2014Article
- Cell cycle checkpoint regulators reach a zillion.Cell cycle (Georgetown, Tex.) · 2013Review
- Carboxy-terminal phosphorylation sites in Cdc25 contribute to enforcement of the DNA damage and replication checkpoints in fission yeast.Current genetics · 2012Article
- Multisite phosphoregulation of Cdc25 activity refines the mitotic entrance and exit switches.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Cell size control in yeast.Current biology : CB · 2012Article
- The Renaissance or the cuckoo clock.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2011Review
- Redundant mechanisms prevent mitotic entry following replication arrest in the absence of Cdc25 hyper-phosphorylation in fission yeast.PloS one · 2011Article
- Regulation of Mih1/Cdc25 by protein phosphatase 2A and casein kinase 1.The Journal of cell biology · 2008Article
- Article
- Joint regulation of the nmt1 promoter and sporulation by Thi1 and Thi5 in Schizosaccharomyces pombe.Current genetics · 2006Article
- Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The DNA replication checkpoint couples the onset of mitosis with the completion of S phase. It is clear that in the fission yeast Schizosaccharomyces pombe, operation of this checkpoint requires maintenance of the inhibitory tyrosyl phosphorylation of Cdc2. Cdc25 phosphatase induces mitosis by dephosphorylating tyrosine 15 of Cdc2. In this report, Cdc25 is shown to accumulate to a very high level in cells arrested in S. This shows that mechanisms which modulate the abundance of Cdc25 are unconnected to the DNA replication checkpoint. Using a Cdc2/cyclin B activation assay, we found that Cdc25 activity increased approximately 10-fold during transit through M phase. Cdc25 was activated by phosphorylations that were dependent on Cdc2 activity in vivo. Cdc25 activation was suppressed in cells arrested in G1 and S. However, Cdc25 was more highly modified and appeared to be somewhat more active in S than in G1. This finding might be connected to the fact that progression from G1 to S increases the likelihood that constitutive Cdc25 overproduction will cause inappropriate mitosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.