ArticleMolecular and cellular biology1996
Fast-muscle-specific DNA-protein interactions occurring in vivo at the human aldolase A M promoter are necessary for correct promoter activity in transgenic mice.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 44 citations in OpenAlex.
- Single-nucleus RNA-seq and FISH identify coordinated transcriptional activity in mammalian myofibers.Nature communications · 2020Article
- DNA methylation assessment from human slow- and fast-twitch skeletal muscle fibers.Journal of applied physiology (Bethesda, Md. : 1985) · 2017Article
- MyoD reprogramming requires Six1 and Six4 homeoproteins: genome-wide cis-regulatory module analysis.Nucleic acids research · 2016Article
- Overexpression of Six1 gene suppresses proliferation and enhances expression of fast-type muscle genes in C2C12 myoblasts.Molecular and cellular biochemistry · 2013Article
- Differentiation and fiber type-specific activity of a muscle creatine kinase intronic enhancer.Skeletal muscle · 2011Article
- Identification of novel transcripts from the porcine MYL1 gene and initial characterization of its promoters.Molecular and cellular biochemistry · 2010Article
- Six1 and Eya1 expression can reprogram adult muscle from the slow-twitch phenotype into the fast-twitch phenotype.Molecular and cellular biology · 2004Article
- Quantitative proteomic identification of six4 as the trex-binding factor in the muscle creatine kinase enhancer.Molecular and cellular biology · 2004Article
- Molecular dissection of DNA sequences and factors involved in slow muscle-specific transcription.Molecular and cellular biology · 2001Article
- The transcription factors MTF-1 and USF1 cooperate to regulate mouse metallothionein-I expression in response to the essential metal zinc in visceral endoderm cells during early development.The EMBO journal · 2001Article
- Functional analysis of the homeodomain protein SIX5.Nucleic acids research · 2000Article
- Human cytomegalovirus IE1 promoter/enhancer drives variable gene expression in all fiber types in transgenic mouse skeletal muscle.BMC genetics · 2000Article
- Fiber-type-specific transcription of the troponin I slow gene is regulated by multiple elements.Molecular and cellular biology · 1999Article
- Expression of myogenin during embryogenesis is controlled by Six/sine oculis homeoproteins through a conserved MEF3 binding site.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Genomic sequences of aldolase C (Zebrin II) direct lacZ expression exclusively in non-neuronal cells of transgenic mice.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- DNA binding and transcriptional activation by the Ski oncoprotein mediated by interaction with NFI.Nucleic acids research · 1997Article
- A combination of MEF3 and NFI proteins activates transcription in a subset of fast-twitch muscles.Molecular and cellular biology · 1997Article
- E-box sites and a proximal regulatory region of the muscle creatine kinase gene differentially regulate expression in diverse skeletal muscles and cardiac muscle of transgenic mice.Molecular and cellular biology · 1996Article
- Independence and interdependence of the three human aldolase A promoters in transgenic mice.Gene expression · 1996Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The human aldolase A tissue-specific M promoter (pM) has served as a model system for identifying pathways that lead to fast-muscle-specialized expression. The current study has delimited the sequences necessary and sufficient for fast-muscle-specific expression in transgenic mice to a short 209-bp fragment extending from bp -164 to +45 relative to the pM transcription start site. Genomic footprinting methods showed that in this proximal region, the same elements that bind muscle nuclear proteins in vitro are involved in DNA-protein interactions in intact muscle nuclei of transgenic mice. Furthermore, these experiments provided the first evidence that different DNA-binding activities exist between slow and fast muscles in vivo. Fast-muscle-specific interactions occur at an element named M1 and at a muscle-specific DNase I-hypersensitive site that was previously detected by in vitro methods. The formation of the muscle-specific DNase I-hypersensitive site reflects binding of proteins to a close element, named M2, which contains a binding site for nuclear factors of the NF1 family. Mutational analysis performed with transgenic mice confirmed the importance of the M1 element for high-level fast-muscle-specific pM activity and suggested that the M2/NF1 element is differently required for correct pM expression in distinct fast muscles. In addition, two other protein binding sites, the MEF3 motif and the USF site, seem to act as stage-specific activators and/or as participants in the establishment of an active chromatin configuration at pM.
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