ArticleMolecular and cellular biology1996
Splicing removes the Caenorhabditis elegans transposon Tc1 from most mutant pre-mRNAs.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 51 citations in OpenAlex.
- Article
- Article
- Article
- Endomembrane-associated RSD-3 is important for RNAi induced by extracellular silencing RNA in both somatic and germ cells of Caenorhabditis elegans.Scientific reports · 2016Article
- Forward and reverse mutagenesis in C. elegans.WormBook : the online review of C. elegans biology · 2014Review
- The Drosophila mojavensis Bari3 transposon: distribution and functional characterization.Mobile DNA · 2014Article
- The spliceosome as a transposon sensor.RNA biology · 2013Review
- Evaluating risks of insertional mutagenesis by DNA transposons in gene therapy.Translational research : the journal of laboratory and clinical medicine · 2013Review
- The N-DRC forms a conserved biochemical complex that maintains outer doublet alignment and limits microtubule sliding in motile axonemes.Molecular biology of the cell · 2013Article
- Identification of transcription start sites of trans-spliced genes: uncovering unusual operon arrangements.RNA (New York, N.Y.) · 2011Article
- Intron creation and DNA repair.Cellular and molecular life sciences : CMLS · 2011Review
- Suppressors of the cdc-25.1(gf)-associated intestinal hyperplasia reveal important maternal roles for prp-8 and a subset of splicing factors in C. elegans.RNA (New York, N.Y.) · 2008Article
- Where do introns come from?PLoS biology · 2008Article
- Alternative splicing and the steady-state ratios of mRNA isoforms generated by it are under strong stabilizing selection in Caenorhabditis elegans.Molecular biology and evolution · 2008Article
- Efficient target-selected mutagenesis in Caenorhabditis elegans: toward a knockout for every gene.Genome research · 2007Article
- Differential expression and function of synaptotagmin 1 isoforms in Caenorhabditis elegans.Molecular and cellular neurosciences · 2007Article
- Structure and transcriptional impact of divergent repetitive elements inserted within Phanerochaete chrysosporium strain RP-78 genes.Molecular genetics and genomics : MGG · 2007Article
- Technology transfer from worms and flies to vertebrates: transposition-based genome manipulations and their future perspectives.Genome biology · 2007Review
- Low impact of germline transposition on the rate of mildly deleterious mutation in Caenorhabditis elegans.Genetics · 2006Article
- Shotgun cloning of transposon insertions in the genome of Caenorhabditis elegans.Comparative and functional genomics · 2004Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The transposable element Tc1 is responsible for most spontaneous mutations that occur in many Caenorhabditis elegans strains. We analyzed the abundance and sequence of mRNAs expressed from five different Tc1 insertions within either hlh-1 (a MyoD homolog) or unc-54 (a myosin heavy chain gene). Each of the mutants expresses substantial quantities of mature mRNA in which most or all of Tc1 has been removed by splicing. Such mRNAs contain small insertions of Tc1 sequences and/or deletions of target gene sequences at the resulting spliced junctions. Most of these mutant mRNAs do not contain premature stop codons, and many are translated in frame to produce proteins that are functional in vivo. The number and variety of splice sites used to remove Tc1 from these mutant pre-mRNAs are remarkable. Two-thirds of the Tc1-containing introns removed from hlh-1 and unc-54 lack either the 5'-GU or AG-3' dinucleotides typically found at the termini of eukaryotic introns. We conclude that splicing to remove Tc1 from mutant pre-mRNAs allows many Tc1 insertions to be phenotypically silent. Such mRNA processing may help Tc1 escape negative selection.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.