ArticleMolecular and cellular biology1996
Activation of transcription by PU.1 requires both acidic and glutamine domains.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- The Myeloid-Specific Transcription Factor PU.1 Upregulates Mannose Receptor Expression but Represses Basal Activity of the HIV-LTR Promoter.Journal of virology · 2022Article
- Article
- Mechanisms governing the pioneering and redistribution capabilities of the non-classical pioneer PU.1.Nature communications · 2020Article
- The transcription factor PU.1 mediates enhancer-promoter looping that is required for IL-1β eRNA and mRNA transcription in mouse melanoma and macrophage cell lines.The Journal of biological chemistry · 2019Article
- The RUNX1-PU.1 axis in the control of hematopoiesis.International journal of hematology · 2015Review
- Transcriptional Factor PU.1 Regulates Decidual C1q Expression in Early Pregnancy in Human.Frontiers in immunology · 2015Article
- Gcn5 is required for PU.1-dependent IL-9 induction in Th9 cells.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
- Mi2beta shows chromatin enzyme specificity by erasing a DNase I-hypersensitive site established by ACF.The Journal of biological chemistry · 2009Article
- Inhibition of IL-1beta transcription by peptides derived from the hCMV IE2 transactivator.Molecular immunology · 2008Article
- PU.1 and pRB interact and cooperate to repress GATA-1 and block erythroid differentiation.Molecular and cellular biology · 2003Article
- Interleukin-3 stimulation of mcl-1 gene transcription involves activation of the PU.1 transcription factor through a p38 mitogen-activated protein kinase-dependent pathway.Molecular and cellular biology · 2003Article
- Inhibition of CBP-mediated protein acetylation by the Ets family oncoprotein PU.1.Molecular and cellular biology · 2002Article
- Control of myeloid-specific integrin alpha Mbeta 2 (CD11b/CD18) expression by cytokines is regulated by Stat3-dependent activation of PU.1.The Journal of biological chemistry · 2002Article
- Evolution of hematopoiesis: Three members of the PU.1 transcription factor family in a cartilaginous fish, Raja eglanteria.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Do lampreys have lymphocytes? The Spi evidence.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Review
- BSAP can repress enhancer activity by targeting PU.1 function.Molecular and cellular biology · 2000Article
- The EICP22 protein of equine herpesvirus 1 physically interacts with the immediate-early protein and with itself to form dimers and higher-order complexes.Journal of virology · 2000Article
- GOOSECOID inhibits erythrocyte differentiation by competing with Rb for PU.1 binding in murine cells.Oncogene · 1999Article
- Cytomegalovirus IE2 protein stimulates interleukin 1beta gene transcription via tethering to Spi-1/PU.1.Molecular and cellular biology · 1999Article
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The B-lymphocyte- and macrophage-specific transcription factor PU.1 is a member of the ets family of proteins. To understand how PU.1 functions as a transcription factor, we initiated a series of experiments to define its activation domain. Using deletion analysis, we showed that the activation domain of PU.1 is located in the amino-terminal half of the protein. Within this region, we identified three acidic subdomains and one glutamine-rich subdomain. The deletion of any of these subdomains resulted in a significant loss in the ability of PU.1 to transactivate in cotransfection studies. Amino acid substitution analysis showed that the activation of transcription by PU.1 requires acidic residues between amino acids 7 and 74 and a group of glutamine residues between amino acids 75 and 84. These data show that PU.1 contains two types of known activation domains and that both are required for maximal transactivation.
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