ArticleMolecular and cellular biology1996
High-frequency illegitimate integration of transfected DNA at preintegrated target sites in a mammalian genome.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 74 citations in OpenAlex.
- High-efficiency and multilocus targeted integration in CHO cells using CRISPR-mediated donor nicking and DNA repair inhibitors.Biotechnology and bioengineering · 2023Article
- TraFo-CRISPR: Enhanced Genome Engineering by Transient Foamy Virus Vector-Mediated Delivery of CRISPR/Cas9 Components.Molecular therapy. Nucleic acids · 2019Article
- MAR-Mediated transgene integration into permissive chromatin and increased expression by recombination pathway engineering.Biotechnology and bioengineering · 2017Article
- Genome engineering with TALENs and ZFNs: repair pathways and donor design.Methods (San Diego, Calif.) · 2014Article
- Mechanism of random integration of foreign DNA in transgenic mice.Transgenic research · 2013Article
- Homologous illegitimate random integration of foreign DNA into the X chromosome of a transgenic mouse line.BMC molecular biology · 2010Article
- Zinc-finger nuclease-driven targeted integration into mammalian genomes using donors with limited chromosomal homology.Nucleic acids research · 2010Article
- Integration of exogenous DNA into mouse embryonic stem cell chromosomes shows preference into genes and frequent modification at junctions.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2010Article
- Article
- A microhomology-mediated break-induced replication model for the origin of human copy number variation.PLoS genetics · 2009Review
- Impact of non-homologous end-joining deficiency on random and targeted DNA integration: implications for gene targeting.Nucleic acids research · 2008Article
- Numt-mediated double-strand break repair mitigates deletions during primate genome evolution.PLoS genetics · 2008Article
- Article
- Characterization of adeno-associated virus genomes isolated from human tissues.Journal of virology · 2005Article
- Targeted mutagenesis using zinc-finger nucleases in Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Detection of integration of plasmid DNA into host genomic DNA following intramuscular injection and electroporation.Gene therapy · 2004Article
- Distinct requirements for Ku in N nucleotide addition at V(D)J- and non-V(D)J-generated double-strand breaks.Nucleic acids research · 2004Article
- Illegitimate DNA integration in mammalian cells.Gene therapy · 2003Review
- Promiscuous patching of broken chromosomes in mammalian cells with extrachromosomal DNA.Nucleic acids research · 2001Article
- Suppression of gene amplification and chromosomal DNA integration by the DNA mismatch repair system.Nucleic acids research · 2001Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
To examine the mechanisms of recombination governing the illegitimate integration of transfected DNA into a mammalian genome, we developed a cell system that selects for integration events in defined genomic regions. Cell lines with chromosomal copies of the 3' portion of the adenine phosphoribosyltransferase (APRT) gene (targets) were established. The 5' portion of the APRT gene, which has no homology to the integrated 3' portion, was then electroporated into the target cell lines, and selection for APRT gene function was applied. The reconstruction of the APRT gene was detected at frequencies ranging from less than 10(-7) to 10(-6) per electroporated cell. Twenty-seven junction sequences between the integrated 5' APRT and its chromosomal target were analyzed. They were found to be randomly distributed in a 2-kb region immediately in front of the 3' portion of the APRT gene. The junctions fell into two main classes: those with short homologies (microhomologies) and those with inserted DNA of uncertain origin. Three long inserts were shown to preexist elsewhere in the genome. Reconstructed cell lines were analyzed for rearrangements at the target site by Southern blotting; a variety of simple and complex rearrangements were detected. Similar analysis of individual clones of the parental cell lines revealed analogous types of rearrangement, indicating that the target sites are unstable. Given the high frequency of integration events at these sites, we speculate that transfected DNA may preferentially integrate at unstable mammalian loci. The results are discussed in relation to possible mechanisms of DNA integration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.