ArticleMolecular and cellular biology1995
Effects of mutations in the Saccharomyces cerevisiae RNA14, RNA15, and PAP1 genes on polyadenylation in vivo.
Article in Molecular and cellular biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 57 citations in OpenAlex.
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- Hydroxyurea and inactivation of checkpoint kinase MEC1 inhibit transcription termination and pre-mRNA cleavage at polyadenylation sites in budding yeast.Scientific reports · 2023Article
- The RNA polymerase II C-terminal domain-interacting domain of yeast Nrd1 contributes to the choice of termination pathway and couples to RNA processing by the nuclear exosome.The Journal of biological chemistry · 2013Article
- Disengaging polymerase: terminating RNA polymerase II transcription in budding yeast.Biochimica et biophysica acta · 2013Review
- RNA degradation in Saccharomyces cerevisae.Genetics · 2012Review
- The yeast RPL9B gene is regulated by modulation between two modes of transcription termination.The EMBO journal · 2012Article
- The spt5 C-terminal region recruits yeast 3' RNA cleavage factor I.Molecular and cellular biology · 2012Article
- The export factor Yra1 modulates mRNA 3' end processing.Nature structural & molecular biology · 2011Article
- The P-loop domain of yeast Clp1 mediates interactions between CF IA and CPF factors in pre-mRNA 3' end formation.PloS one · 2011Article
- Assembly of an export-competent mRNP is needed for efficient release of the 3'-end processing complex after polyadenylation.Molecular and cellular biology · 2009Article
- Cordycepin interferes with 3' end formation in yeast independently of its potential to terminate RNA chain elongation.RNA (New York, N.Y.) · 2009Article
- The role of the putative 3' end processing endonuclease Ysh1p in mRNA and snoRNA synthesis.RNA (New York, N.Y.) · 2008Article
- The role of Rat1 in coupling mRNA 3'-end processing to transcription termination: implications for a unified allosteric-torpedo model.Genes & development · 2006Article
- A nuclear surveillance pathway for mRNAs with defective polyadenylation.Molecular and cellular biology · 2005Article
- The role of the yeast cleavage and polyadenylation factor subunit Ydh1p/Cft2p in pre-mRNA 3'-end formation.Nucleic acids research · 2003Article
- Yhh1p/Cft1p directly links poly(A) site recognition and RNA polymerase II transcription termination.The EMBO journal · 2002Article
- T7 RNA polymerase-directed transcripts are processed in yeast and link 3' end formation to mRNA nuclear export.RNA (New York, N.Y.) · 2002Article
- Probabilistic prediction of Saccharomyces cerevisiae mRNA 3'-processing sites.Nucleic acids research · 2002Article
- Rna15 interaction with the A-rich yeast polyadenylation signal is an essential step in mRNA 3'-end formation.Molecular and cellular biology · 2001Article
- Mpe1, a zinc knuckle protein, is an essential component of yeast cleavage and polyadenylation factor required for the cleavage and polyadenylation of mRNA.Molecular and cellular biology · 2001Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
The RNA14 and RNA15 gene products have been implicated in a variety of cellular processes. Mutations in these genes lead to faster decay of some mRNAs and yield extracts that are deficient in cleavage and polyadenylation in vitro. These results suggest that the RNA14 and RNA15 gene products may be involved in both adenylation and deadenylation in vivo. To explore the roles of these gene products in vivo, we examined the site of adenylation and the rate of deadenylation for individual mRNAs in rna14 and rna15 mutant strains. We observed that the rates of deadenylation are not affected by lesions in either the RNA14 or the RNA15 gene. This result suggests that the proteins encoded by these genes are not involved in regulation of the deadenylation rate. In contrast, we observed that the site of adenylation for the ACT1 transcript can be altered in these mutants. Interestingly, we also observed that mutation of the poly(A) polymerase gene altered the site of ACT1 polyadenylation. These observations suggest that the RNA14, RNA15, and PAP1 proteins are involved in poly(A) site choice. This alteration in poly(A) site choice in the rna14 mutant can be corrected by the ssm4 suppressor, indicating that this suppression acts at the level of polyadenylation and not by slowing mRNA degradation.
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