ArticleMolecular and cellular biology1995
Functional reconstruction of trans regulation of the Ultrabithorax promoter by the products of two antagonistic genes, trithorax and Polycomb.
Article in Molecular and cellular biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 46 citations in OpenAlex.
- Organ transformation by environmental disruption of protein integrity and epigenetic memory in Drosophila.PLoS biology · 2024Article
- DNA sequence models of genome-wide Drosophila melanogaster Polycomb binding sites improve generalization to independent Polycomb Response Elements.Nucleic acids research · 2019Article
- Three classes of response elements for human PRC2 and MLL1/2-Trithorax complexes.Nucleic acids research · 2018Article
- Loss of the insulator protein CTCF during nematode evolution.BMC molecular biology · 2009Article
- A double-bromodomain protein, FSH-S, activates the homeotic gene ultrabithorax through a critical promoter-proximal region.Molecular and cellular biology · 2007Article
- Transcriptional network controlled by the trithorax-group gene ash2 in Drosophila melanogaster.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- pipsqueak encodes a factor essential for sequence-specific targeting of a polycomb group protein complex.Molecular and cellular biology · 2002Article
- Essential role of Drosophila Hdac1 in homeotic gene silencing.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Review
- Structure of a polycomb response element and in vitro binding of polycomb group complexes containing GAGA factor.Molecular and cellular biology · 2000Article
- Trithorax and ASH1 interact directly and associate with the trithorax group-responsive bxd region of the Ultrabithorax promoter.Molecular and cellular biology · 1999Article
- Trithorax- and Polycomb-group response elements within an Ultrabithorax transcription maintenance unit consist of closely situated but separable sequences.Molecular and cellular biology · 1999Article
- Mutant alleles of the Drosophila trithorax gene produce common and unusual homeotic and other developmental phenotypes.Genetics · 1999Article
- Binding of trithorax and Polycomb proteins to the bithorax complex: dynamic changes during early Drosophila embryogenesis.The EMBO journal · 1998Article
- Characterization of interactions between the mammalian polycomb-group proteins Enx1/EZH2 and EED suggests the existence of different mammalian polycomb-group protein complexes.Molecular and cellular biology · 1998Article
- The polycomb group protein complex of Drosophila melanogaster has different compositions at different target genes.Molecular and cellular biology · 1997Article
- RING1 is associated with the polycomb group protein complex and acts as a transcriptional repressor.Molecular and cellular biology · 1997Article
- Co-localization of Polycomb protein and GAGA factor on regulatory elements responsible for the maintenance of homeotic gene expression.The EMBO journal · 1997Article
- Hunchback-independent silencing of late Ubx enhancers by a Polycomb Group Response Element.The EMBO journal · 1996Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Maintenance of the "on-off" state of Drosophila homeotic genes in Antennapedia and bithorax complexes requires activities of the trithorax and Polycomb groups of genes. To identify cis-acting sequences for functional reconstruction of regulation by both trithorax and Polycomb, we examined the expression patterns of several Ubx-lacZ transgenes that carry upstream fragments corresponding to a region of approximately 50 kb. A 14.5-kb fragment from the postbithorax/bithoraxoid region of Ultrabithorax exhibited proper regulation by both trithorax and Polycomb in the embryonic central nervous system. Using a Drosophila haploid cell line for transient expression, we found that trithorax or Polycomb can function independently through this upstream fragment to activate or repress the Ultrabithorax promoter, respectively. Studies of deletion mutants of trithorax and Polycomb demonstrated that trithorax-dependent activation requires the central zinc-binding domain, while Polycomb-dependent repression requires the intact chromodomain. In addition, trithorax-dependent activity can be abrogated by increasing the amount of Polycomb, suggesting a competitive interaction between the products of trithorax and Polycomb. Deletion analysis of this fragment demonstrated that a 440-bp fragment contains response elements for both trithorax and Polycomb. Furthermore, we showed that the integrity of the proximal promoter region is essential for trithorax-dependent activation, implicating a long-range interaction for promoter activation.
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