ArticleThe Journal of cell biology1995
An amino-terminal extension is required for the secretion of chick agrin and its binding to extracellular matrix.
Article in The Journal of cell biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 130 citations in OpenAlex.
- Defective perlecan-associated basement membrane regeneration and altered modulation of transforming growth factor beta in corneal fibrosis.Cellular and molecular life sciences : CMLS · 2022Review
- Congenital Disorders of Deficiency in Glycosaminoglycan Biosynthesis.Frontiers in genetics · 2021Review
- Extracellular signal-regulated kinases 1 and 2 regulate neuromuscular junction and myofiber phenotypes in mammalian skeletal muscle.Neuroscience letters · 2020Review
- Basement membranes in the cornea and other organs that commonly develop fibrosis.Cell and tissue research · 2018Review
- Dynein disruption perturbs post-synaptic components and contributes to impaired MuSK clustering at the NMJ: implication in ALS.Scientific reports · 2016Article
- The heparan sulfate proteoglycan agrin contributes to barrier properties of mouse brain endothelial cells by stabilizing adherens junctions.Cell and tissue research · 2014Article
- Recycling of acetylcholine receptors at ectopic postsynaptic clusters induced by exogenous agrin in living rats.Developmental biology · 2014Article
- Solubilization and identification of hen eggshell membrane proteins during different times of chicken embryo development using the proteomic approach.The protein journal · 2013Article
- Article
- The coxsackievirus-adenovirus receptor reveals complex homophilic and heterophilic interactions on neural cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- Laminin deposition in the extracellular matrix: a complex picture emerges.Journal of cell science · 2009Article
- An interdomain disulfide bridge links the NtA and first FS domain in agrin.Protein science : a publication of the Protein Society · 2009Article
- Scaffold-forming and Adhesive Contributions of Synthetic Laminin-binding Proteins to Basement Membrane Assembly.The Journal of biological chemistry · 2009Article
- Rescuing Z+ agrin splicing in Nova null mice restores synapse formation and unmasks a physiologic defect in motor neuron firing.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Neural agrin changes the electrical properties of developing human skeletal muscle cells.Cellular and molecular neurobiology · 2009Article
- Agrin expression during synaptogenesis induced by traumatic brain injury.Journal of neurotrauma · 2008Article
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- Defects in eye development in transgenic mice overexpressing the heparan sulfate proteoglycan agrin.Developmental biology · 2007Article
- Local induction of acetylcholine receptor clustering in myotube cultures using microfluidic application of agrin.Biophysical journal · 2006Article
- HIV-1-infected blood mononuclear cells form an integrin- and agrin-dependent viral synapse to induce efficient HIV-1 transcytosis across epithelial cell monolayer.Molecular biology of the cell · 2005Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Agrin is an extracellular matrix (ECM) protein with a calculated relative molecular mass of more than 200 kD that induces the aggregation of acetylcholine receptors (AChRs) at the neuromuscular junction. This activity has been mapped to its COOH terminus. In an attempt to identify the functions of the NH2-terminal end, we have now characterized full-length chick agrin. We show that chick agrin encoded by a previously described cDNA is not secreted from transfected cells. Secretion is achieved with a construct that includes an additional 350 bp derived from the 5' end of chick agrin mRNA. Recombinant agrin is a heparan sulfate proteoglycan (HSPG) of more than 400 kD with glycosaminoglycan side chains attached only to the NH2-terminal half. Endogenous agrin in tissue homogenates also has an apparent molecular mass of > 400 kD. While the amino acid sequence encoded by the 350-bp extension has no homology to published rat agrin, it includes a stretch of 15 amino acids that is 80% identical to a previously identified bovine HSPG. The extension is required for binding of agrin to ECM. AChR aggregates induced by recombinant agrin that includes the extension are considerably smaller than those induced by agrin fragments, suggesting that binding of agrin to ECM modulates the size of receptor clusters. In addition, we found a site encoding seven amino acids at the NH2-terminal end of agrin that is alternatively spliced. While motor neurons express the splice variant with the seven amino acid long insert, muscle cells mainly synthesize isoforms that lack this insert. In conclusion, the cDNAs described here code for chick agrin that has all the characteristics previously allocated to endogenous agrin.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.