ArticleThe Journal of cell biology1995
Actin filaments in yeast are unstable in the absence of capping protein or fimbrin.
Article in The Journal of cell biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 86 citations in OpenAlex.
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- Plastin 3 in health and disease: a matter of balance.Cellular and molecular life sciences : CMLS · 2021Review
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- Computational model of polarized actin cables and cytokinetic actin ring formation in budding yeast.Cytoskeleton (Hoboken, N.J.) · 2015Article
- Actin cable distribution and dynamics arising from cross-linking, motor pulling, and filament turnover.Molecular biology of the cell · 2014Article
- The availability of filament ends modulates actin stochastic dynamics in live plant cells.Molecular biology of the cell · 2014Article
- The plant-specific actin binding protein SCAB1 stabilizes actin filaments and regulates stomatal movement in Arabidopsis.The Plant cell · 2011Article
- Arabidopsis FIMBRIN5, an actin bundling factor, is required for pollen germination and pollen tube growth.The Plant cell · 2010Article
- F-actin dynamics in Neurospora crassa.Eukaryotic cell · 2010Article
- How tropomyosin regulates lamellipodial actin-based motility: a combined biochemical and reconstituted motility approach.The EMBO journal · 2010Article
- Comprehensive and quantitative analysis of yeast deletion mutants defective in apical and isotropic bud growth.Current genetics · 2009Article
- Endocytosis optimizes the dynamic localization of membrane proteins that regulate cortical polarity.Cell · 2007Article
- The yeast actin cytoskeleton: from cellular function to biochemical mechanism.Microbiology and molecular biology reviews : MMBR · 2006Review
- Actin depolymerization is sufficient to induce programmed cell death in self-incompatible pollen.The Journal of cell biology · 2006Article
- Article
- Capping protein binding to actin in yeast: biochemical mechanism and physiological relevance.The Journal of cell biology · 2004Article
- Signal-mediated depolymerization of actin in pollen during the self-incompatibility response.The Plant cell · 2002Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Many actin-binding proteins affect filament assembly in vitro and localize with actin in vivo, but how their molecular actions contribute to filament assembly in vivo is not understood well. We report here that capping protein (CP) and fimbrin are both important for actin filament assembly in vivo in Saccharomyces cerevisiae, based on finding decreased actin filament assembly in CP and fimbrin mutants. We have also identified mutations in actin that enhance the CP phenotype and find that those mutants also have decreased actin filament assembly in vivo. In vitro, actin purified from some of these mutants is defective in polymerization or binding fimbrin. These findings support the conclusion that CP acts to stabilize actin filaments in vivo. This conclusion is particularly remarkable because it is the opposite of the conclusion drawn from recent studies in Dictyostelium (Hug, C., P.Y. Jay, I. Reddy, J.G. McNally, P.C. Bridgman, E.L. Elson, and J.A. Cooper. 1995. Cell. 81:591-600). In addition, we find that the unpolymerized pool of actin in yeast is very small relative to that found in higher cells, which suggests that actin filament assembly is less dynamic in yeast than higher cells.
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