ArticleThe Journal of cell biology1995
Anterograde and retrograde traffic between the rough endoplasmic reticulum and the Golgi complex.
Article in The Journal of cell biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 102 citations in OpenAlex.
- Models of Intracellular Transport: Contradictions and Current Understanding.Sub-cellular biochemistry · 2026Review
- Sorting and Export of Proteins at the Endoplasmic Reticulum.Cold Spring Harbor perspectives in biology · 2023Review
- Vesicular and uncoated Rab1-dependent cargo carriers facilitate ER to Golgi transport.Journal of cell science · 2020Article
- Probing the Heterogeneity of Protein Kinase Activation in Cells by Super-resolution Microscopy.ACS nano · 2017Article
- Mitotic Golgi disassembly is required for bipolar spindle formation and mitotic progression.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- A genetically specified connectomics approach applied to long-range feeding regulatory circuits.Nature neuroscience · 2014Article
- The TBC/RabGAP Armus coordinates Rac1 and Rab7 functions during autophagy.Developmental cell · 2013Article
- Glycosylation disorders of membrane trafficking.Glycoconjugate journal · 2013Review
- The kiss-and-run model of intra-Golgi transport.International journal of molecular sciences · 2012Article
- Interaction between FIP5 and SNX18 regulates epithelial lumen formation.The Journal of cell biology · 2011Article
- Endophilin drives the fast mode of vesicle retrieval in a ribbon synapse.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Organisation of human ER-exit sites: requirements for the localisation of Sec16 to transitional ER.Journal of cell science · 2009Article
- Assembly, organization, and function of the COPII coat.Histochemistry and cell biology · 2008Review
- Neurexin Ibeta and neuroligin are localized on opposite membranes in mature central synapses.Journal of neurochemistry · 2007Article
- Analysis of de novo Golgi complex formation after enzyme-based inactivation.Molecular biology of the cell · 2007Article
- Tetanus toxin is internalized by a sequential clathrin-dependent mechanism initiated within lipid microdomains and independent of epsin1.The Journal of cell biology · 2006Article
- Golgi enzymes are enriched in perforated zones of golgi cisternae but are depleted in COPI vesicles.Molecular biology of the cell · 2004Article
- Annexin 11 is required for midbody formation and completion of the terminal phase of cytokinesis.The Journal of cell biology · 2004Article
- Ultrastructural analysis of transitional endoplasmic reticulum and pre-Golgi intermediates: a highway for cars and trucks.Histochemistry and cell biology · 2003Article
- Membrane targeting of Rab GTPases is influenced by the prenylation motif.Molecular biology of the cell · 2003Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The transfer of newly synthesized membrane proteins moving from the rough endoplasmic reticulum (RER) to the Golgi complex has been studied by electron microscopy in HEp-2 cells transfected with cDNAs for chimeric proteins. These proteins consist of a reporter enzyme, horseradish peroxidase (HRP), anchored to the transmembrane domains of two integral membrane proteins, the transferrin receptor and sialyl-transferase. The chimeras are distributed throughout the nuclear envelope, RER, vesicular tubular clusters (VTCs) and a network of tubules in the cis-Golgi area. At 20 degrees C tubules containing chimera connect the RER to the VTCs and to the cis-Golgi network. On transfer to 37 degrees C in the presence of dithiothreitol (DTT), the chimeras are seen to move from the RER and through the Golgi stack. With this temperature shift the direct connections with the RER are lost and free vesicles form; some of these vesicles contain HRP reaction product which is much more concentrated than in the adjacent RER while others lack reaction product entirely. In cells expressing SSHRPKDEL, DAB reaction product remains distributed throughout the RER, the VTCs, and the cis-Golgi network for prolonged periods in the presence of DTT and almost all of the vesicles which form at 37 degrees C are DAB-positive. Together these observations demonstrate that all three chimeras are transported from the RER to the cis-Golgi in free, 40-60-nm vesicles at 37 degrees C. They also suggest that the retrograde traffic which carries SSHRPKDEL back to the RER is probably mediated by vesicles with a similar morphology but which, in cells expressing membrane-anchored chimeras, lack detectable reaction product.
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