ArticleThe Journal of cell biology1995
Stable, detyrosinated microtubules function to localize vimentin intermediate filaments in fibroblasts.
Article in The Journal of cell biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed, 163 citations in OpenAlex.
- Paclitaxel compromises nuclear integrity in interphase through SUN2-mediated cytoskeletal coupling.Journal of cell science · 2026Article
- The unexpected structure and dynamics of vimentin networks.The Journal of cell biology · 2025Article
- Structural basis for α-tubulin-specific and modification state-dependent glutamylation.Nature chemical biology · 2024Article
- The microtubule signature in cardiac disease: etiology, disease stage, and age dependency.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2023Article
- Novel chalcone-derived pyrazoles as potential therapeutic agents for the treatment of non-small cell lung cancer.Scientific reports · 2022Article
- Pathophysiological Role of Vimentin Intermediate Filaments in Lung Diseases.Frontiers in cell and developmental biology · 2022Review
- Vimentin intermediate filaments stabilize dynamic microtubules by direct interactions.Nature communications · 2021Article
- Mechanical and Non-Mechanical Functions of Filamentous and Non-Filamentous Vimentin.BioEssays : news and reviews in molecular, cellular and developmental biology · 2020Review
- Filamentous actin disorganization and absence of apical ectoplasmic specialization disassembly during spermiation upon interference with retinoid signaling†.Biology of reproduction · 2020Article
- The Tubulin Code in Microtubule Dynamics and Information Encoding.Developmental cell · 2020Review
- Microtubules: Evolving roles and critical cellular interactions.Experimental biology and medicine (Maywood, N.J.) · 2019Review
- Rudhira/BCAS3 couples microtubules and intermediate filaments to promote cell migration for angiogenic remodeling.Molecular biology of the cell · 2019Article
- Unbiased pattern analysis reveals highly diverse responses of cytoskeletal systems to cyclic straining.PloS one · 2019Article
- Microtubules Provide a Viscoelastic Resistance to Myocyte Motion.Biophysical journal · 2018Article
- Exogenous growth factors enhance the expression of cola1, cola3, and Elastin in fibroblasts via activating MAPK signaling pathway.Molecular and cellular biochemistry · 2018Article
- Rice intermediate filament, OsIF, stabilizes photosynthetic machinery and yield under salinity and heat stress.Scientific reports · 2018Article
- Microtubule mechanics in the working myocyte.The Journal of physiology · 2017Review
- Identification of a novel transcript isoform of the TTLL12 gene in human cancers.Oncology reports · 2016Article
- Mapping intracellular mechanics on micropatterned substrates.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Vimentin Intermediate Filaments Template Microtubule Networks to Enhance Persistence in Cell Polarity and Directed Migration.Cell systems · 2016Article
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Authors and funding
2 authors at 1 institution in 1 country.
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Abstract
Separate populations of microtubules (MTs) distinguishable by their level of posttranslationally modified tubulin subunits and by their stability in vivo have been described. In polarized 3T3 cells at the edge of an in vitro wound, we have found a striking preferential coalignment of vimentin intermediate filaments (IFs) with detyrosinated MTs (Glu MTs) rather than with the bulk of the MTs, which were tyrosinated MTs (Tyr MTs). Vimentin IFs were not stabilizing the Glu MTs since collapse of the IF network to a perinuclear location, induced by microinjection of monoclonal anti-IF antibody, had no noticeable effect on the array of Glu MTs. To test whether Glu MTs may affect the organization of IFs we regrew MTs in cells that had been treated with nocodazole to depolymerize all the MTs and to collapse IFs; the reextension of IFs into the lamella lagged behind the rapid regrowth of Tyr MTs, but was correlated with the slower reformation of Glu MTs. Similar realignment of IFs with newly formed Glu MTs was observed in serum-starved cells treated with either serum or taxol to induce the formation of Glu MTs. Next, we microinjected affinity purified antibodies specific for Glu tubulin (polyclonal SG and monoclonal 4B8) and specific for Tyr tubulin (polyclonal W2 and monoclonal YL1/2) into 3T3 cells. Both injected SG and 4B8 antibodies labeled the subset of endogenous Glu MTs; W2 and YL1/2 antibodies labeled virtually all of the cytoplasmic MTs. Injection of SG or 4B8 resulted in the collapse of IFs to a perinuclear region. This collapse was comparable to that observed after complete MT depolymerization by nocodazole. Injection of W2, YL1/2, or nonspecific control IgGs did not result in collapse of the IFs. Taken together, these results show that Glu MTs localize IFs in migrating 3T3 fibroblasts and suggest that detyrosination of tubulin acts as a signal for the recruitment of vimentin IFs to MTs.
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