ArticleThe Journal of cell biology1995
Dictyostelium myosin I double mutants exhibit conditional defects in pinocytosis.
Article in The Journal of cell biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
77 citing papers in PubMed, 196 citations in OpenAlex.
- The RNA-binding protein RNP1A is essential and interacts with contractility kit proteins to facilitate cell mechanics.Journal of cell science · 2026Article
- Modulation of Filopodial Myosin Function.bioRxiv : the preprint server for biology · 2025Article
- Procedures to Measure Dictyostelium Phagocytosis and Macropinocytosis.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Article
- Basic-hydrophobic sites are localized in conserved positions inside and outside of PH domains and affect localization ofMolecular biology of the cell · 2020Article
- Application of advances in endocytosis and membrane trafficking to drug delivery.Advanced drug delivery reviews · 2020Review
- Human myosin 1e tail but not motor domain replaces fission yeast Myo1 domains to support myosin-I function during endocytosis.Experimental cell research · 2019Article
- High-throughput Measurement of Dictyostelium discoideum Macropinocytosis by Flow Cytometry.Journal of visualized experiments : JoVE · 2018Article
- The physiological regulation of macropinocytosis duringJournal of cell science · 2018Article
- Myosin-1C uses a novel phosphoinositide-dependent pathway for nuclear localization.EMBO reports · 2018Article
- Article
- Structure of the Single-lobe Myosin Light Chain C in Complex with the Light Chain-binding Domains of Myosin-1C Provides Insights into Divergent IQ Motif Recognition.The Journal of biological chemistry · 2016Article
- Myosin-I molecular motors at a glance.Journal of cell science · 2016Review
- Selective localization of myosin-I proteins in macropinosomes and actin waves.Cytoskeleton (Hoboken, N.J.) · 2016Article
- Class I myosins have overlapping and specialized functions in left-right asymmetric development in Drosophila.Genetics · 2015Article
- Three-dimensional balance of cortical tension and axial contractility enables fast amoeboid migration.Biophysical journal · 2015Article
- Shaping the intestinal brush border.The Journal of cell biology · 2014Review
- Structure of the small Dictyostelium discoideum myosin light chain MlcB provides insights into MyoB IQ motif recognition.The Journal of biological chemistry · 2014Article
- Requirements for Hirano body formation.Eukaryotic cell · 2014Article
- ER sheet persistence is coupled to myosin 1c-regulated dynamic actin filament arrays.Molecular biology of the cell · 2014Article
17 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
The functional relationship between three Dictyostelium myosin Is, myoA, myoB, and myoC, has been examined through the creation of double mutants. Two double mutants, myoA-/B- and myoB-/C-, exhibit similar conditional defects in fluid-phase pinocytosis. Double mutants grown in suspension culture are significantly impaired in their ability to take in nutrients from the medium, whereas they are almost indistinguishable from wild-type and single mutant strains when grown on a surface. The double mutants are also found to internalize gp126, a 116-kD membrane protein, at a slower rate than either the wild-type or single mutant cells. Ultrastructural analysis reveals that both double mutants possess numerous small vesicles, in contrast to the wild-type or myosin I single mutants that exhibit several large, clear vacuoles. The alterations in fluid and membrane internalization in the suspension-grown double mutants, coupled with the altered vesicular profile, suggest that these cells may be compromised during the early stages of pinocytosis, a process that has been proposed to occur via actin-based cytoskeletal rearrangements. Scanning electron microscopy and rhodamine-phalloidin staining indicates that the myosin I double mutants appear to extend a larger number of actin-filled structures, such as filopodia and crowns, than wild-type cells. Rhodamine-phalloidin staining of the F-actin cytoskeleton of these suspension-grown cells also reveals that the double mutant cells are delayed in the rearrangement of cortical actin-rich structures upon adhesion to a substrate. We propose that myoA, myoB, and myoC play roles in controlling F-actin filled membrane projections that are required for pinosome internalization in suspension.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.