ArticleThe Journal of cell biology1995
Chromosomes initiate spindle assembly upon experimental dissolution of the nuclear envelope in grasshopper spermatocytes.
Article in The Journal of cell biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 61 citations in OpenAlex.
- Male meiotic spindle features that efficiently segregate paired and lagging chromosomes.eLife · 2020Article
- Micromanipulation of Chromosomes in Insect Spermatocytes.Journal of visualized experiments : JoVE · 2018Article
- Metaphase Spindle Assembly.Biology · 2017Review
- Chromosomal attachments set length and microtubule number in the Saccharomyces cerevisiae mitotic spindle.Molecular biology of the cell · 2014Article
- CaM kinase II initiates meiotic spindle depolymerization independently of APC/C activation.The Journal of cell biology · 2008Article
- Kinetochore-microtubule interactions during cell division.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2004Review
- Contribution of noncentrosomal microtubules to spindle assembly in Drosophila spermatocytes.PLoS biology · 2004Article
- Spindle formation in Aspergillus is coupled to tubulin movement into the nucleus.Molecular biology of the cell · 2003Article
- Spindle assembly and cytokinesis in the absence of chromosomes during Drosophila male meiosis.The Journal of cell biology · 2003Article
- Male meiotic spindle lengths in normal and mutant arabidopsis cells.Plant physiology · 2001Article
- Regulation of Op18 during spindle assembly in Xenopus egg extracts.The Journal of cell biology · 2001Article
- Skeletor, a novel chromosomal protein that redistributes during mitosis provides evidence for the formation of a spindle matrix.The Journal of cell biology · 2000Article
- Mutation of a Drosophila gamma tubulin ring complex subunit encoded by discs degenerate-4 differentially disrupts centrosomal protein localization.Genes & development · 2000Article
- Spindle assembly and mitosis without centrosomes in parthenogenetic Sciara embryos.The Journal of cell biology · 1998Article
- Microinjection of antibody to Mad2 protein into mammalian cells in mitosis induces premature anaphase.The Journal of cell biology · 1998Article
- Mitotic spindle poles are organized by structural and motor proteins in addition to centrosomes.The Journal of cell biology · 1997Article
- Spindle assembly in Xenopus egg extracts: respective roles of centrosomes and microtubule self-organization.The Journal of cell biology · 1997Article
- Chromosomal proteins and cytokinesis: patterns of cleavage furrow formation and inner centromere protein positioning in mitotic heterokaryons and mid-anaphase cells.The Journal of cell biology · 1997Article
- Sliding of antiparallel microtubules drives bipolarization of monoastral spindles.Cytoskeleton (Hoboken, N.J.)Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Chromosomes are known to enhance spindle microtubule assembly in grasshopper spermatocytes, which suggested to us that chromosomes might play an essential role in the initiation of spindle formation. Chromosomes might, for example, activate other spindle components such as centrosomes and tubulin subunits upon the breakdown of the nuclear envelope. We tested this possibility in living grasshopper spermatocytes. We ruptured the nuclear envelope during prophase, which prematurely exposed the centrosomes to chromosomes and nuclear sap. Spindle assembly was promptly initiated. In contrast, assembly of the spindle was completely inhibited if the nucleus was mechanically removed from a late prophase cell. Other experiments showed that the trigger for spindle assembly is associated with the chromosomes; other constituents of the nucleus cannot initiate spindle assembly in the absence of the chromosomes. The initiation of spindle assembly required centrosomes as well as chromosomes. Extracting centrosomes from late prophase cells completely inhibited spindle assembly after dissolution of the nuclear envelope. We conclude that the normal formation of a bipolar spindle in grasshopper spermatocytes is regulated by chromosomes. A possible explanation is an activator, perhaps a chromosomal protein (Yeo, J.-P., F. Alderuccio, and B.-H. Toh. 1994a. Nature (Lond.). 367: 288-291), that promotes and stabilizes the assembly of astral microtubules and thus promotes assembly of the spindle.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.