ArticleClinical & experimental metastasis1996
EGF stimulates lamellipod extension in metastatic mammary adenocarcinoma cells by an actin-dependent mechanism.
Article in Clinical & experimental metastasis, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
61 citing papers in PubMed, 144 citations in OpenAlex.
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- The core autophagy protein ATG9A controls dynamics of cell protrusions and directed migration.The Journal of cell biology · 2022Article
- Macrophages enhance 3D invasion in a breast cancer cell line by induction of tumor cell tunneling nanotubes.Cancer reports (Hoboken, N.J.) · 2019Article
- CD206Scientific reports · 2019Article
- Tunneling nanotubes, a novel mode of tumor cell-macrophage communication in tumor cell invasion.Journal of cell science · 2019Article
- Computational model of wound healing: EGF secreted by fibroblasts promotes delayed re-epithelialization of epithelial keratinocytes.Integrative biology : quantitative biosciences from nano to macro · 2018Article
- The Role of Actin Dynamics and Actin-Binding Proteins Expression in Epithelial-to-Mesenchymal Transition and Its Association with Cancer Progression and Evaluation of Possible Therapeutic Targets.BioMed research international · 2018Review
- The glycolytic enzyme phosphofructokinase-1 assembles into filaments.The Journal of cell biology · 2017Article
- Blood vessel endothelium-directed tumor cell streaming in breast tumors requires the HGF/C-Met signaling pathway.Oncogene · 2017Article
- A Phosphatidic Acid (PA) conveyor system of continuous intracellular transport from cell membrane to nucleus maintains EGF receptor homeostasis.Oncotarget · 2016Article
- Cancer stem cells and tumor-associated macrophages: a roadmap for multitargeting strategies.Oncogene · 2016Review
- Epidermal growth factor promotes a mesenchymal over an amoeboid motility of MDA-MB-231 cells embedded within a 3D collagen matrixEuropean physical journal plus · 2016Article
- The multiple roles of epidermal growth factor repeat O-glycans in animal development.Glycobiology · 2015Review
- Structures of human phosphofructokinase-1 and atomic basis of cancer-associated mutations.Nature · 2015Article
- Real-time monitoring of cell protrusion dynamics by impedance responses.Scientific reports · 2015Article
- Spatial regulation of tumor cell protrusions by RhoC.Cell adhesion & migration · 2014Review
- An in vitro one-dimensional assay to study growth factor-regulated tumor cell-macrophage interaction.Methods in molecular biology (Clifton, N.J.) · 2014Article
- Spatial regulation of RhoC activity defines protrusion formation in migrating cells.Journal of cell science · 2013Article
1 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Changes in lamellipod extension and chemotaxis in response to EGF were analysed for MTLn3 cells (a metastatic cell line derived from the 13762NF rat mammary adenocarcinoma). Addition of EGF produced a cessation of ruffling followed by extension of hyaline lamellipods containing increased amounts of F-actin at the growing edge. A non-metastatic cell line (MTC) derived from the same tumor did not show such responses. Lamellipod extension was maximal within 5 min, followed by retraction and resumption of ruffling. Maximal area increases due to lamellipod extension occurred at about 5 nM EGF. Chemotactic and chemokinetic responses, measured using a microchemotaxis chamber, were also greatest at 5 nM. Cytochalasin D inhibited EGF-stimulated responses including lamellipod extension, increases in F-actin in lamellipods, and chemotaxis. Nocodazole affected chemotaxis at higher concentrations but not EGF-induced lamellipod extension. We conclude that polymerization of F-actin at the leading edges of lamellipods is necessary for extension of lamellipods and chemotaxis of MTLn3 cells in response to EGF. The motility and chemotaxis responses of this metastatic cell line have strong similarities to those seen in well-characterized chemotactic cells such as Dictyostelium and neutrophils.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.