ArticleThe Journal of clinical investigation1993
Restricted immunoglobulin VH usage and VDJ combinations in the human response to Haemophilus influenzae type b capsular polysaccharide. Nucleotide sequences of monospecific anti-Haemophilus antibodies and polyspecific antibodies cross-reacting with self antigens.
Article in The Journal of clinical investigation, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 98 citations in OpenAlex.
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- Germline-encoded neutralization of a Staphylococcus aureus virulence factor by the human antibody repertoire.Nature communications · 2016Article
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- In-Depth Assessment of Within-Individual and Inter-Individual Variation in the B Cell Receptor Repertoire.Frontiers in immunology · 2015Article
- Immune markers characteristic for asymptomatically infected and diseased Entamoeba histolytica individuals and their relation to sex.BMC infectious diseases · 2014Article
- Decreased mutation frequencies among immunoglobulin G variable region genes during viremic HIV-1 infection.PloS one · 2014Article
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- Effects of adjuvants on IgG subclasses elicited by virus-like particles.Journal of translational medicine · 2012Article
- Clonal expansion of immunoglobulin M+CD27+ B cells in HCV-associated mixed cryoglobulinemia.Blood · 2008Article
- Correlation of molecular characteristics, isotype, and in vitro functional activity of human antipneumococcal monoclonal antibodies.Infection and immunity · 2006Article
- Specificity and overlap in gene segment-defined antibody repertoires.BMC genomics · 2005Article
- Specificity and diversity of antibodies to Mycobacterium tuberculosis arabinomannan.Clinical and diagnostic laboratory immunology · 2003Article
- Antibody repertoires in infants and adults: effects of T-independent and T-dependent immunizations.Springer seminars in immunopathology · 2001Review
- Immunoglobulin E antibodies of atopic individuals exhibit a broad usage of VH-gene families.Immunology · 2000Article
- Additive effect of neutralizing antibody and antiviral drug treatment in preventing virus escape and persistence.Journal of virology · 2000Article
- Factors that influence formation of B cell repertoire.Immunologic research · 2000Review
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
To examine the human antibody repertoire generated against a biologically significant antigen we have obtained sequences of heavy chain variable region genes (IgVH) from 15 monoclonal antibodies specific for the capsular polysaccharide of Haemophilus influenzae type b (Hib PS). All VH segments are members of the VH3 family and 9 of 15 are members of the smaller VH3b subfamily. Restriction is evident by the shared use of certain VDJ joints in independent hybridomas from different subjects. Two hybridomas generated from the same subject demonstrate identical heavy chain variable region gene sequences but differ in isotype and rearrange alternative light chain variable region genes (IgVL), suggesting that in a normal immune response, a single pre-B cell clone may use different light chain rearrangements and give rise to progeny capable of reacting with antigen. Using a polymerase chain reaction assay optimized to detect base pair differences among VH genes we demonstrate that at least a portion of expressed anti-Hib PS VH genes have undergone somatic mutation. Anti-Hib PS heavy chain genes are homologous to VH segments encoding autoantibodies and two hybridomas secrete anti-Hib PS antibody that cross-reacts with self antigens (double-stranded DNA and single-stranded DNA). Comparison of VH regions of self-reactive and monospecific anti-Hib PS Ab demonstrates no consistent structural feature correlating with fine antigen specificity. These data demonstrate significant restriction in VH usage and VDJ recombination in the anti-Hib PS response and confirm that autoantibodies may be elicited during normal immune responses.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.