ArticleTransgenic research1993
An SAR sequence containing 395 bp DNA fragment mediates enhanced, gene-dosage-correlated expression of a chimaeric heat shock gene in transgenic tobacco plants.
Article in Transgenic research, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 85 citations in OpenAlex.
- Insights Into Genetic and Molecular Elements for Transgenic Crop Development.Frontiers in plant science · 2020Article
- Review
- Minimizing the unpredictability of transgene expression in plants: the role of genetic insulators.Plant cell reports · 2012Review
- Functional analysis of BnMAR element in transgenic tobacco plants.Molecular biology reports · 2011Article
- Size of AT(n) insertions in promoter region modulates Gmhsp17.6-L mRNA transcript levels.Journal of biomedicine & biotechnology · 2010Article
- The influence of matrix attachment regions on transgene expression in Arabidopsis thaliana wild type and gene silencing mutants.Plant molecular biology · 2007Article
- Matrix attachment region from the chicken lysozyme locus reduces variability in transgene expression and confers copy number-dependence in transgenic rice plants.Plant cell reports · 2005Article
- Isolation and characterisation of three moss-derived beta-tubulin promoters suitable for recombinant expression.Current genetics · 2005Article
- TM2, a novel strong matrix attachment region isolated from tobacco, increases transgene expression in transgenic rice calli and plants.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2005Article
- The transcriptional enhancer of the pea plastocyanin gene associates with the nuclear matrix and regulates gene expression through histone acetylation.The Plant cell · 2003Article
- Analysis of trans-silencing interactions using transcriptional silencers of varying strength and targets with and without flanking nuclear matrix attachment regions.Transgenic research · 2003Article
- Molecular structure and regulatory potential of a T-DNA integration site in petunia.Transgenic research · 2003Article
- Suppression of transgene silencing by matrix attachment regions in maize: a dual role for the maize 5' ADH1 matrix attachment region.The Plant cell · 2002Article
- Combinatorial interaction of cis elements specifies the expression of the Arabidopsis AtHsp90-1 gene.Plant physiology · 2002Article
- Use of matrix attachment regions (MARs) to minimize transgene silencing.Plant molecular biology · 2000Review
- Prediction of transgene integration by noninvasive bioluminescent screening of microinjected bovine embryos.Transgenic research · 1998Article
- A/T-rich sequences act as quantitative enhancers of gene expression in transgenic tobacco and potato plants.Plant molecular biology · 1998Article
- Human matrix attachment regions insulate transgene expression from chromosomal position effects in Drosophila melanogaster.Molecular and cellular biology · 1998Article
- The heat shock cognate 80 gene of tomato is flanked by matrix attachment regions.Plant molecular biology · 1996Article
- High-level transgene expression in plant cells: effects of a strong scaffold attachment region from tobacco.The Plant cell · 1996Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A 395 bp fragment located downstream from the soybean heat shock gene Gmhsp 17.6-L exhibits several characteristics of scaffold attachment region (SAR) sequences. It contains matrix consensus elements, a topoisomerase II binding sequence and it associates with the isolated nuclear scaffold of soybean in vitro. Chimaeric genes containing the SARL fragment either at one side (5' or 3') or at both sides of a heat shock promoter-regulated beta-glucuronidase reporter gene were constructed. A five- to nine-fold increase of heat-inducible beta-glucuronidase activity was observed in transgenic tobacco plants containing constructs with SARL fragments either at both sides or with at least one SARL copy located upstream from the reporter gene. The gene copy number is positively correlated with the level of heat-inducible reporter gene activity in these plants but positional effects are not entirely eliminated. Thus, SAR sequences may potentially be used to increase gene expression, via as yet unknown mechanisms, and to reduce adverse effects on the expression of multiple gene copies in transgenic plants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.