ArticleMolecular & general genetics : MGG1993
Identification and characterization of a chlorate-resistant mutant of Arabidopsis thaliana with mutations in both nitrate reductase structural genes NIA1 and NIA2.
Article in Molecular & general genetics : MGG, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 111 papers.
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Who cites it
111 citing papers in PubMed.
- Article
- Functional divergence and symbiotic significance of nitrate reductase isoforms in Medicago truncatula.Plant physiology · 2026Article
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- The Maize ZmbHLH118 Transcription Factor Regulates Vacuolar Nitrate Loading by the NOAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Article
- Transcriptional regulation modulates nitrate uptake and utilization ofFrontiers in plant science · 2026Article
- DSK2-mediated degradation of F-box protein LAO1 and class I TCPs modulates the nitrogen starvation response.EMBO reports · 2025Article
- Evolutionary-conserved RLF, a cytochrome bThe New phytologist · 2025Article
- Nitrate activates an MKK3-dependent MAPK module via NLP transcription factors in Arabidopsis.The Plant journal : for cell and molecular biology · 2025Article
- Nitric oxide participates in sucrose-TOR signaling during meristem activation in Arabidopsis thaliana.Planta · 2024Article
- Suppression of SMXL4 and SMXL5 confers enhanced thermotolerance through promoting HSFA2 transcription in Arabidopsis.The Plant cell · 2024Article
- The interplay between cell wall integrity and cell cycle progression in plants.Plant molecular biology · 2023Review
- Non-Targeted Metabolomic Analysis ofMetabolites · 2023Article
- The transcription factors, STOP1 and TCP20, are required for root system architecture alterations in response to nitrate deficiency.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Moonlighting Arabidopsis molybdate transporter 2 family and GSH-complex formation facilitate molybdenum homeostasis.Communications biology · 2023Article
- The Arabidopsis transcription factor NLP2 regulates early nitrate responses and integrates nitrate assimilation with energy and carbon skeleton supply.The Plant cell · 2023Article
- Fast-track transformation and genome editing in Brachypodium distachyon.Plant methods · 2023Article
- Nitric Oxide Acts as a Key Signaling Molecule in Plant Development under Stressful Conditions.International journal of molecular sciences · 2023Review
- Genome-Wide Analysis of theInternational journal of molecular sciences · 2023Article
- Cloning and characterization of nitrate reductase gene in kelp Saccharina japonica (Laminariales, Phaeophyta).BMC plant biology · 2023Article
51 more citing papers are in PubMed but not listed here.
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2 authors.
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Abstract
Mutant plants defective in the assimilation of nitrate can be selected by their resistance to the herbicide chlorate. In Arabidopsis thaliana, mutations at any one of nine distinct loci confer chlorate resistance. Only one of the CHL genes, CHL3, has been shown genetically to be a nitrate reductase (NR) structural gene (NIA2) even though two NR genes (NIA1 and NIA2) have been cloned from the Arabidopsis genome. Plants in which the NIA2 gene has been deleted retain only 10% of the wild-type shoot NR activity and grow normally with nitrate as the sole nitrogen source. Using mutagenized seeds from the NIA2 deletion mutant and a modified chlorate selection protocol, we have identified the first mutation in the NIA1 NR structural gene. nia1, nia2 double mutants have only 0.5% of wild-type shoot NR activity and display very poor growth on media with nitrate as the only form of nitrogen. The nia1-1 mutation is a single nucleotide substitution that converts an alanine to a threonine in a highly conserved region of the molybdenum cofactor-binding domain of the NR protein. These results show that the NIA1 gene encodes a functional NR protein that contributes to the assimilation of nitrate in Arabidopsis.
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