ArticleThe Journal of cell biology1993
The cytoplasmic tail of CD44 is required for basolateral localization in epithelial MDCK cells but does not mediate association with the detergent-insoluble cytoskeleton of fibroblasts.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 101 citations in OpenAlex.
- CD44 Intracellular Domain: A Long Tale of a Short Tail.Cancers · 2023Review
- Transcriptome analysis of renal ischemia/reperfusion (I/R) injury in BAFF and BAFF-R deficient mice.PloS one · 2023Article
- CD44-Targeted Nanocarrier for Cancer Therapy.Frontiers in pharmacology · 2021Review
- Ping-Pong-Tumor and Host in Pancreatic Cancer Progression.Frontiers in oncology · 2019Review
- CD44/CD44v6 a Reliable Companion in Cancer-Initiating Cell Maintenance and Tumor Progression.Frontiers in cell and developmental biology · 2018Review
- Rhomboid intramembrane protease RHBDL4 triggers ER-export and non-canonical secretion of membrane-anchored TGFα.Scientific reports · 2016Article
- CD44, Hyaluronan, the Hematopoietic Stem Cell, and Leukemia-Initiating Cells.Frontiers in immunology · 2015Review
- Article
- HAS3-induced accumulation of hyaluronan in 3D MDCK cultures results in mitotic spindle misorientation and disturbed organization of epithelium.Histochemistry and cell biology · 2012Article
- CD44: can a cancer-initiating cell profit from an abundantly expressed molecule?Nature reviews. Cancer · 2011Review
- The differences between the localizations of MUC1, MUC5AC, MUC6 and osteopontin in quail proventriculus and gizzard may be a reflection of functional differences of stomach parts.Journal of anatomy · 2010Article
- Functional roles of short sequence motifs in the endocytosis of membrane receptors.Frontiers in bioscience (Landmark edition) · 2009Review
- Protective effect of hyaluronate on oxidative DNA damage in WI-38 and A549 cells.International journal of oncology · 2008Article
- Directional sensing of a phorbol ester gradient requires CD44 and is regulated by CD44 phosphorylation.Oncogene · 2006Article
- A new spreadsheet method for the analysis of bivariate flow cytometric data.BMC cell biology · 2004Article
- CD44 in cancer progression: adhesion, migration and growth regulation.Journal of molecular histology · 2004Review
- Initial steps of Shigella infection depend on the cholesterol/sphingolipid raft-mediated CD44-IpaB interaction.The EMBO journal · 2002Article
- Cytoskeleton-dependent membrane domain segregation during neutrophil polarization.Molecular biology of the cell · 2001Article
- Ca(2+)/calmodulin-dependent protein kinase mediates the phosphorylation of CD44 required for cell migration on hyaluronan.The Biochemical journal · 2001Article
- The CEACAM1-L glycoprotein associates with the actin cytoskeleton and localizes to cell-cell contact through activation of Rho-like GTPases.Molecular biology of the cell · 2000Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
A number of recent reports on the trafficking of receptor proteins in MDCK epithelial cells have provided evidence that delivery to the basolateral domain requires a specific targeting sequence and that deletion of this sequence results in constitutive expression on the apical surface. To date, these studies have concentrated on receptors which are competent for internalization via the clathrin coated pits. We have examined the localization of a resident plasma membrane protein by transfecting human CD44 into MDCK cells. Using human specific and cross-species reactive antibodies, we show that in MDCK cells both the endogenous and transfected wild-type CD44 are found on the basolateral surface where they are restricted to the lateral domain. Deletion of the CD44 cytoplasmic tail reduces the half life of this mutant protein and causes it to be expressed both on the apical surface and to a significant extent within the cell. We have also used biochemical and morphological analysis to investigate the interaction of CD44 with the cytoskeleton in detergent extracted cells. Strikingly different extraction results were obtained between epithelial and fibroblast cells. However, there is no difference in the Triton X-100 solubility of the transfected wild-type and tail-less CD44 in fibroblasts and both forms of the protein remain associated with the cortical cytoskeleton after Triton X-100 extraction. These results demonstrate that the sequence present in the cytoplasmic domain of CD44 responsible for its distribution in epithelial cells is functionally and spatially separate from the ability of this protein to associate with the cytoskeleton.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.