ArticleThe Journal of cell biology1993
Biogenesis of peroxisomes: isolation and characterization of two distinct peroxisomal populations from normal and regenerating rat liver.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 93 citations in OpenAlex.
- Visualisation of peroxisomes: a journey through seven decades.Histochemistry and cell biology · 2026Review
- Germ cell-specific deletion ofJournal of biomedical research · 2023Article
- ACOX-driven peroxisomal heterogeneity and functional compartmentalization in brown adipocytes of hypothyroid rats.Royal Society open science · 2023Article
- Hypothyroidism Intensifies Both Canonic and theCells · 2021Article
- The peroxisome: an update on mysteries 2.0.Histochemistry and cell biology · 2018Review
- Article
- Transient complex peroxisomal interactions: A new facet of peroxisome dynamics in mammalian cells.Communicative & integrative biology · 2012Article
- Postfixation detergent treatment liberates the membrane modelling protein Pex11β from peroxisomal membranes.Histochemistry and cell biology · 2012Article
- Localization of the pre-squalene segment of the isoprenoid biosynthetic pathway in mammalian peroxisomes.Histochemistry and cell biology · 2007Article
- Peroxisomes are present in murine spermatogonia and disappear during the course of spermatogenesis.Histochemistry and cell biology · 2006Article
- Peroxisome division in the yeast Yarrowia lipolytica is regulated by a signal from inside the peroxisome.The Journal of cell biology · 2003Article
- Fusion of small peroxisomal vesicles in vitro reconstructs an early step in the in vivo multistep peroxisome assembly pathway of Yarrowia lipolytica.The Journal of cell biology · 2000Article
- Peroxisome distribution along the crypt-villus axis of the guinea pig small intestine.Molecular and cellular biochemistry · 2000Article
- Arabidopsis 22-kilodalton peroxisomal membrane protein. Nucleotide sequence analysis and biochemical characterization.Plant physiology · 1999Article
- The cytosolic DnaJ-like protein djp1p is involved specifically in peroxisomal protein import.The Journal of cell biology · 1998Article
- Overexpression of Pex15p, a phosphorylated peroxisomal integral membrane protein required for peroxisome assembly in S.cerevisiae, causes proliferation of the endoplasmic reticulum membrane.The EMBO journal · 1997Article
- In vitro systems in the study of peroxisomal protein import.Experientia · 1996Review
- Multiple PEX genes are required for proper subcellular distribution and stability of Pex5p, the PTS1 receptor: evidence that PTS1 protein import is mediated by a cycling receptor.The Journal of cell biology · 1996Article
- Redox-sensitive homodimerization of Pex11p: a proposed mechanism to regulate peroxisomal division.The Journal of cell biology · 1996Article
- Contributions of the immunogold technique to investigation of the biology of peroxisomes.Histochemistry and cell biology · 1996Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
According to Poole et al. (1970, J. Cell Biol. 45:408-415), newly synthesized peroxisomal proteins are incorporated uniformly into peroxisomes (PO) of different size classes, suggesting that rat hepatic PO form a homogeneous population. There is however increasing cytochemical and biochemical evidence that PO in rat liver are heterogenous, undergoing significant modulations in shape and size in process of PO morphogenesis (Yamamoto and Fahimi, 1987. J. Cell Biol. 105:713-722). In the present study, the kinetics of incorporation of newly synthesized proteins into distinct PO-subpopulations have been studied using short-term in vivo labeling (5-90 min). Two distinct "heavy" and "light" crude PO fractions were prepared by differential pelleting from normal and regenerating liver, and highly purified PO were subsequently isolated by density-dependent metrizamide gradient centrifugation according to Völkl and Fahimi (1985. Eur. J. Biochem. 149:257-265). The peroxisomal fractions banded at 1.20 and 1.24 g x cm-3. They differed in their mean diameters and form-factors and particularly in respect to the activity of beta-oxidation enzymes which was higher in the "light" PO. Whereas the "light" PO exhibited a single immunoreactive band with the antibody to the 70-kD peroxisomal membrane protein the "heavy" PO contained an additional (68 kD) band. In pulse-labeling experiments "light" PO showed clearly a higher initial rate of incorporation than the "heavy" PO. The relative specific activity in the "heavy" PO fraction, however increased progressively reaching that of "light" PO by 90 min. These observations provide evidence for the existence of different PO populations in rat liver which differ in their morphological and biochemical properties as well as in their rates of incorporation of new proteins.
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