ArticleThe EMBO journal1993
GCR1, a transcriptional activator in Saccharomyces cerevisiae, complexes with RAP1 and can function without its DNA binding domain.
Article in The EMBO journal, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed.
- Cis- and trans-regulatory factors contributing to divergent activity of the TDH3 promoter in Saccharomyces yeast.Molecular biology and evolution · 2026Article
- Article
- Mapping transcription factor binding sites by learning UV damage fingerprints.Nucleic acids research · 2025Article
- Review
- A novel aminotransferase gene and its regulator acquired in Saccharomyces by a horizontal gene transfer event.BMC biology · 2023Article
- Evolution of transcription factor binding through sequence variations and turnover of binding sites.Genome research · 2022Article
- A High-Copy Suppressor Screen Reveals a Broad Role of Prefoldin-like Bud27 in the TOR Signaling Pathway inGenes · 2022Article
- Differential activation mechanisms of two isoforms of Gcr1 transcription factor generated from spliced and un-spliced transcripts in Saccharomyces cerevisiae.Nucleic acids research · 2021Article
- Promoter Architecture and Promoter Engineering inMetabolites · 2020Review
- Quantitative analysis of transcription factor binding and expression using calling cards reporter arrays.Nucleic acids research · 2020Article
- Homotypic cooperativity and collective binding are determinants of bHLH specificity and function.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Impaired GCR1 transcription resulted in defective inositol levels, vacuolar structure and autophagy in Saccharomyces cerevisiae.Current genetics · 2019Article
- The molecular biology of fruity and floral aromas in beer and other alcoholic beverages.FEMS microbiology reviews · 2019Review
- Posttranscriptional Regulation of Gcr1 Expression and Activity Is Crucial for Metabolic Adjustment in Response to Glucose Availability.Molecular cell · 2016Article
- Genome-wide studies of telomere biology in budding yeast.Microbial cell (Graz, Austria) · 2014Review
- Massively parallel synthetic promoter assays reveal the in vivo effects of binding site variants.Genome research · 2013Article
- Rap1 relocalization contributes to the chromatin-mediated gene expression profile and pace of cell senescence.Genes & development · 2013Article
- Review
- Article
- Distribution of short interstitial telomere motifs in two plant genomes: putative origin and function.BMC plant biology · 2010Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
In Saccharomyces cerevisiae, efficient expression of glycolytic and translational component genes requires two DNA binding proteins, RAP1 (which binds to UASRPG) and GCR1 (which binds to the CT box). We generated deletions in GCR1 to test the validity of several different models for GCR1 function. We report here that the C-terminal half of GCR1, which includes the domain required for DNA binding to the CT box in vitro, can be removed without affecting GCR1-dependent transcription of either the glycolytic gene ADH1 or the translational component genes TEF1 and TEF2. We have also identified an activation domain within a segment of the GCR1 protein (the N-terminal third) that is essential for in vivo function. RAP1 and GCR1 can be co-immunoprecipitated from whole cell extracts, suggesting that they form a complex in vivo. The data are most consistent with a model in which GCR1 is attracted to DNA through contact with RAP1.
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