ArticleThe American journal of pathology1993
Detection of clonal immunoglobulin gene rearrangements by polymerase chain reaction amplification and single-strand conformational polymorphism analysis.
Article in The American journal of pathology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 55 citations in OpenAlex.
- Experience with the BIOMED-2 standardized polymerase chain reaction protocol for immunoglobulin and T- cell receptor clonality analysis in the Instituto Nacional de Cancerología, ColombiaBiomedica : revista del Instituto Nacional de Salud · 2022Article
- Molecular Testing of Lymphoproliferative Disorders: Current Status and Perspectives.Journal of pathology and translational medicine · 2017Review
- Clonality analysis of lymphoid proliferations using the BIOMED-2 clonality assays: a single institution experience.Radiology and oncology · 2014Article
- Clonal immunoglobulin heavy chain and T-cell receptor γ gene rearrangements in primary gastric lymphoma.World journal of gastroenterology · 2013Article
- Review
- Rapid and accurate detection of monoclonal immunoglobulin heavy chain gene rearrangement by DNA melting curve analysis in the LightCycler System.The Journal of molecular diagnostics : JMD · 2002Article
- Article
- Detection of immunoglobulin kappa light chain rearrangements by polymerase chain reaction. An improved method for detecting clonal B-cell lymphoproliferative disorders.The American journal of pathology · 1999Article
- Establishment of a novel B cell clonality analysis using single-strand conformation polymorphism of immunoglobulin light chain messenger signals.The American journal of pathology · 1998Article
- Identification of clonally expanded T cells in rheumatoid arthritis using a sequence enrichment nuclease assay.The Journal of clinical investigation · 1996Article
- Detection of clonal immunoglobulin heavy chain (IgH) gene rearrangement by nested PCR amplification and single strand conformational polymorphism (SSCP) analysis.The American journal of pathology · 1994Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Analysis of immunoglobulin gene rearrangements by Southern blotting is a sensitive and specific method for detecting B cell malignancies but requires a relatively large amount of intact DNA. It cannot be utilized in many cases where only a small amount of tissue is available or where the tissue has been fixed. This report demonstrates that polymerase chain reaction (PCR) amplification in conjunction with single-strand conformational polymorphism (SSCP) analysis can be utilized to detect clonal immunoglobulin heavy chain (IgH) gene rearrangements. IgH gene rearrangements from a series of frozen or formalin-fixed B cell malignancies were PCR-amplified using oligonucleotide primers, based upon consensus sequences in the IgH variable and joining regions. Analysis of the single-stranded PCR products on nondenaturing polyacrylamide gels revealed discrete SSCPs corresponding to the malignant B cells. These SSCPs were detectable when the malignant cells represented as few as 0.2% of the total mononuclear cells in peripheral blood. PCR amplification in conjunction with SSCP analysis thus provides a sensitive and specific method to detect clonal IgH rearrangements from minute amounts of fresh, frozen, or fixed tissue.
Indexed as
Identifiers
8506952PMC1886985W165585736What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.