ArticleProceedings of the National Academy of Sciences of the United States of America1993
Modulation of the interaction between G-actin and thymosin beta 4 by the ATP/ADP ratio: possible implication in the regulation of actin dynamics.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 156 citations in OpenAlex.
- Thymosin β4 promotes zebrafish Mauthner axon regeneration by facilitating actin polymerization through binding to G-actin.BMC biology · 2024Article
- The role of the cytoskeleton in fibrotic diseases.Frontiers in cell and developmental biology · 2024Review
- Thymosin β4 is essential for thrombus formation by controlling the G-actin/F-actin equilibrium in platelets.Haematologica · 2022Article
- The Actin Regulators Involved in the Function and Related Diseases of Lymphocytes.Frontiers in immunology · 2022Review
- Towards a structural understanding of the remodeling of the actin cytoskeleton.Seminars in cell & developmental biology · 2020Review
- Profilin choreographs actin and microtubules in cells and cancer.International review of cell and molecular biology · 2020Article
- Primary Cilia, Ciliogenesis and the Actin Cytoskeleton: A Little Less Resorption, A Little More Actin Please.Frontiers in cell and developmental biology · 2020Review
- Steroidal glycoalkaloids fromPeerJ · 2019Article
- Reconsidering an active role for G-actin in cytoskeletal regulation.Journal of cell science · 2018Review
- The C-terminal dimerization motif of cyclase-associated protein is essential for actin monomer regulation.The Biochemical journal · 2016Article
- Internetwork competition for monomers governs actin cytoskeleton organization.Nature reviews. Molecular cell biology · 2016Article
- Structural basis of thymosin-β4/profilin exchange leading to actin filament polymerization.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- The role of cyclase-associated protein in regulating actin filament dynamics - more than a monomer-sequestration factor.Journal of cell science · 2013Review
- ATP-dependent regulation of actin monomer-filament equilibrium by cyclase-associated protein and ADF/cofilin.The Biochemical journal · 2013Article
- There is more than one way to model an elephant. Experiment-driven modeling of the actin cytoskeleton.Biophysical journal · 2013Review
- Controlling the cortical actin motor.Protoplasma · 2012Review
- CAS-1, a C. elegans cyclase-associated protein, is required for sarcomeric actin assembly in striated muscle.Journal of cell science · 2012Article
- Prediction and dissection of widely-varying association rate constants of actin-binding proteins.PLoS computational biology · 2012Article
- Conformational dynamics of actin: effectors and implications for biological function.Cytoskeleton (Hoboken, N.J.) · 2010Review
- Modeling capping protein FRAP and CALI experiments reveals in vivo regulation of actin dynamics.Cytoskeleton (Hoboken, N.J.) · 2010Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The interaction of G-actin with thymosin beta 4 (T beta 4), the major G-actin-sequestering protein in motile and proliferating cells, has been analyzed in vitro. T beta 4 is found to have a 50-fold higher affinity for MgATP-actin than for MgADP-actin. These results imply that in resting platelets and neutrophils, actin is sequestered by T beta 4 as MgATP-G-actin. Kinetic experiments and theoretical calculations demonstrate that this ATP/ADP dependence of T beta 4 affinity for G-actin can generate a mechanism of desequestration of G-actin by ADP, in the presence of physiological concentrations of T beta 4 (approximately 0.1 mM). The desequestration of G-actin by ADP is kinetically enhanced by profilin, which accelerates the dissociation of ATP from G-actin. Whether a local drop in the ATP/ADP ratio can allow local, transient desequestration and polymerization of actin either close to the plasma membrane, following platelet or neutrophil stimulation, or behind the Listeria bacterium in the host cell, while the surrounding cytoplasm contains sequestered ATP-G-actin, is an open issue raised by the present work.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.