ArticleNucleic acids research1993
Far upstream regions of class II MHC Ea are necessary for position-independent, copy-dependent expression of Ea transgene.
Article in Nucleic acids research, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 57 citations in OpenAlex.
- Regulation of major histocompatibility complex class II gene expression, genetic variation and disease.Genes and immunity · 2010Review
- CFTR expression from a BAC carrying the complete human gene and associated regulatory elements.Journal of cellular and molecular medicine · 2009Article
- Transcription-coupled deposition of histone modifications during MHC class II gene activation.Nucleic acids research · 2007Article
- Locus control regions.Blood · 2002Review
- Murine MHC class II locus control region drives expression of human beta-glucocerebrosidase in antigen presenting cells of transgenic mice.Gene therapy · 1999Article
- Dividing the empire: boundary chromatin elements delimit the territory of enhancers.The EMBO journal · 1999Review
- Severe B cell deficiency and disrupted splenic architecture in transgenic mice expressing the E41K mutated form of Bruton's tyrosine kinase.The EMBO journal · 1998Article
- Retinoblastoma protein expression facilitates chromatin remodeling at the HLA-DRA promoter.Nucleic acids research · 1997Article
- Correction of the X-linked immunodeficiency phenotype by transgenic expression of human Bruton tyrosine kinase under the control of the class II major histocompatibility complex Ea locus control region.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Position independent expression and developmental regulation is directed by the beta myosin heavy chain gene's 5' upstream region in transgenic mice.Nucleic acids research · 1995Article
- Delivery of DNA into mammalian cells by receptor-mediated endocytosis and gene therapy.Molecular biotechnology · 1995Review
- Sequencing of an upstream region of the human HLA-DRA gene containing X' and Y' boxes.Nucleic acids research · 1995Article
- Contrasting effects of alpha and beta globin regulatory elements on chromatin structure may be related to their different chromosomal environments.The EMBO journal · 1995Article
- Analysis of a 70 kb segment of DNA containing the human zeta and alpha-globin genes linked to their regulatory element (HS-40) in transgenic mice.Nucleic acids research · 1994Article
- Dissection of the locus control function located on the chicken lysozyme gene domain in transgenic mice.Nucleic acids research · 1994Article
- The 5' flanking region of the rat LAP (C/EBP beta) gene can direct high-level, position-independent, copy number-dependent expression in multiple tissues in transgenic mice.Nucleic acids research · 1994Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The chromatin upstream of the class II MHC Ea gene contains specific, DNase I hypersensitive (DH) sites (groups I-V), overlapping and extending the promoter proximal and distal control regions. To determine whether the Ea DH groups I-V define a functionally important chromatin domain or locus control region (LCR), we have used wild type Ead gene constructs to generate transgenic mouse lines from strains that do not express an endogenous Ea gene product. Constructs contained either DH groups I-V 'Longs' or DH groups I-II 'Shorts', of the hypersensitive sites defined within 20 kb 5' of Ea. We show that position-independent, copy number-dependent expression of the Ead gene occurs only with the Long construct (8/8 transgenic mouse lines, over a range of copy numbers, 1-30 copies); in contrast, the Short constructs are subject to position-dependent effects. This suggests that the region delineated by Ea DH groups I-II is necessary but not sufficient as an LCR, which requires the presence of the upstream regions containing DH III-V for complete position-independent, copy number-dependent expression. These results introduce an immunologically-important, putative LCR which can be used to target genes to cells of the B cell lineage, as well as to other class II MHC expressing cells, and highlight the importance of chromatin structure analysis as a means to locate DNA regions of regulatory interest which are dispersed over a large distance.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.