ArticleJournal of clinical microbiology1993
Serotype identification of Actinobacillus pleuropneumoniae by arbitrarily primed polymerase chain reaction.
Article in Journal of clinical microbiology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 46 citations in OpenAlex.
- Optimal regimens based on PK/PD cutoff evaluation of ceftiofur against Actinobacillus pleuropneumoniae in swine.BMC veterinary research · 2020Article
- Multiplex PCR assay for detection of Actinobacillus pleuropneumoniae, Pasteurella multocida and Haemophilus parasuis in lungs of pigs from a slaughterhouse.Folia microbiologica · 2010Article
- DNA microarray-based identification and typing of Actinobacillus pleuropneumoniae.Canadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2009Article
- Detection and identification of Actinobacillus pleuropneumoniae serotypes 1, 2, and 8 by multiplex PCR.Journal of clinical microbiology · 2004Article
- Evaluation of a multiplex PCR test for simultaneous identification and serotyping of Actinobacillus pleuropneumoniae serotypes 2, 5, and 6.Journal of clinical microbiology · 2003Article
- Mutations in the lspA1 and lspA2 genes of Haemophilus ducreyi affect the virulence of this pathogen in an animal model system.Infection and immunity · 2003Article
- Characterization of Haemophilus ducreyi cdtA, cdtB, and cdtC mutants in in vitro and in vivo systems.Infection and immunity · 2001Article
- Identification of the znuA-encoded periplasmic zinc transport protein of Haemophilus ducreyi.Infection and immunity · 1999Article
- Pasteurellaceae isolated from tonsillar samples of commercially-reared American bison (Bison bison).Canadian journal of veterinary research = Revue canadienne de recherche veterinaire · 1999Article
- Genomic relatedness among Actinobacillus pleuropneumoniae field strains of sterotypes 1 and 5 isolated from healthy and diseased pigs.Canadian journal of veterinary research = Revue canadienne de recherche veterinaire · 1999Article
- Characterization of a WaaF (RfaF) homolog expressed by Haemophilus ducreyi.Infection and immunity · 1999Article
- Involvement of the Haemophilus ducreyi gmhA gene product in lipooligosaccharide expression and virulence.Infection and immunity · 1998Article
- Characterization of serologically nontypeable Actinobacillus actinomycetemcomitans isolates.Journal of clinical microbiology · 1998Article
- Detection and identification of Actinobacillus pleuropneumoniae serotype 5 by multiplex PCR.Journal of clinical microbiology · 1998Article
- DNA fingerprinting of medically important microorganisms by use of PCR.Clinical microbiology reviews · 1994Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rapid and accurate determination of the Actinobacillus pleuropneumoniae serotype involved in a disease outbreak is important both in limiting the severity of an outbreak and for tracing the source of the infecting organism. This study describes the use of arbitrarily primed polymerase chain reaction (AP-PCR) as a rapid, precise, and genetically based procedure to identify A. pleuropneumoniae. AP-PCR amplification of bacterial genomic DNA results in specific DNA profiles, which can be used to differentiate currently recognized serotypes. This technique is especially useful for identifying previously nontypeable and serologically cross-reactive A. pleuropneumoniae field isolates. Consecutive passages of isolates on different media, freezing, and subsequent infection of pigs did not alter the AP-PCR genomic profile. We propose the use of M13 and T3-T7 oligodeoxynucleotide primers for diagnostic and epidemiological identification of A. pleuropneumoniae by AP-PCR techniques.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.