ArticleJournal of clinical microbiology1993
Application of polymerase chain reaction to fingerprinting Aspergillus fumigatus by random amplification of polymorphic DNA.
Article in Journal of clinical microbiology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- Review
- Comparison of Two Highly Discriminatory Typing Methods to AnalyzeFrontiers in microbiology · 2018Article
- Diversity, molecular phylogeny and fingerprint profiles of airborne Aspergillus species using random amplified polymorphic DNA.World journal of microbiology & biotechnology · 2016Article
- Genotyping bacterial and fungal pathogens using sequence variation in the gene for the CCA-adding enzyme.BMC microbiology · 2016Article
- Article
- Whole-genome comparison of Aspergillus fumigatus strains serially isolated from patients with aspergillosis.Journal of clinical microbiology · 2014Article
- Molecular characterization of Aspergillus infections in an Iranian educational hospital using RAPD-PCR method.Iranian journal of basic medical sciences · 2014Article
- Molecular epidemiology of Aspergillus fumigatus: an in-depth genotypic analysis of isolates involved in an outbreak of invasive aspergillosis.Journal of clinical microbiology · 2011Article
- Multilocus sequence typing of the pathogenic fungus Aspergillus fumigatus.Journal of clinical microbiology · 2007Article
- Comparison of two highly discriminatory molecular fingerprinting assays for analysis of multiple Aspergillus fumigatus isolates from patients with invasive aspergillosis.Journal of clinical microbiology · 2007Article
- Use of a novel panel of nine short tandem repeats for exact and high-resolution fingerprinting of Aspergillus fumigatus isolates.Journal of clinical microbiology · 2005Article
- Differentiation of Aspergillus ustus strains by random amplification of polymorphic DNA.Journal of clinical microbiology · 2002Article
- Comparison of restriction fragment length polymorphism, microsatellite length polymorphism, and random amplification of polymorphic DNA analyses for fingerprinting Aspergillus fumigatus isolates.Journal of clinical microbiology · 2001Article
- The ins and outs of DNA fingerprinting the infectious fungi.Clinical microbiology reviews · 2000Review
- Aspergillus fumigatus and aspergillosis.Clinical microbiology reviews · 1999Review
- Use of the 6-methylsalicylic-acid-synthase gene as a discriminating marker between Aspergillus terreus and Aspergillus flavipes.Folia microbiologica · 1999Article
- The evolutionary biology and population genetics underlying fungal strain typing.Clinical microbiology reviews · 1999Review
- Microsatellite markers for typing Aspergillus fumigatus isolates.Journal of clinical microbiology · 1998Article
- Molecular typing of environmental and patient isolates of Aspergillus fumigatus from various hospital settings.Journal of clinical microbiology · 1998Article
- Molecular epidemiological study of Aspergillus fumigatus in a bone marrow transplantation unit by PCR amplification of ribosomal intergenic spacer sequences.Journal of clinical microbiology · 1998Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
A new method for fingerprinting Aspergillus fumigatus by random amplification of polymorphic DNA (RAPD) by using single primers with arbitrary sequences is described. Five primers were examined with 19 isolates from six patients with aspergilloma as well as with A. fumigatus NCPF 2109. Two of the primers (GCT GGT GG and GCG CAC GG, 5' to 3') gave adequate discrimination between isolates, generating five and six types, respectively. Combination of the results obtained with each of these two primers generated 12 types. This compares very favorably with immunoblot fingerprinting and XbaI-generated restriction fragment length polymorphisms on the same isolates. Typeability and reproducibility were good with RAPD, and RAPD was less labor-intensive than immunoblot fingerprinting. RAPD typing results suggested that aspergillomas sometimes contain isolates of more than one type.
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