ArticleThe Journal of cell biology1993
Accumulation of PDGF B and cell-binding forms of PDGF A in the extracellular matrix.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 90 citations in OpenAlex.
- Detection of platelet-derived growth factor (PDGF)-AA in actively healing human wounds treated with recombinant PDGF-BB and absence of PDGF in chronic nonhealing wounds.The Journal of clinical investigation · 1995Trial
- Integrated Genomics Reveals Convergent Transcriptomic Networks Underlying Chronic Obstructive Pulmonary Disease and Idiopathic Pulmonary Fibrosis.American journal of respiratory and critical care medicine · 2016Article
- Article
- Role of platelet-derived growth factors in physiology and medicine.Genes & development · 2008Review
- Redesigned and chemically-modified hammerhead ribozymes with improved activity and serum stability.BMC chemical biology · 2004Article
- Differential binding of platelet-derived growth factor isoforms to glycosaminoglycans.Histochemistry and cell biology · 2003Article
- Identifying ribozyme-accessible sites using NUH triplet-targeting gapmers.Nucleic acids research · 2001Article
- Regulation of the spatial organization of mesenchymal connective tissue: effects of cell-associated versus released isoforms of platelet-derived growth factor.The American journal of pathology · 1999Article
- Loop III region of platelet-derived growth factor (PDGF) B-chain mediates binding to PDGF receptors and heparin.The Biochemical journal · 1998Article
- Tumor cell and connective tissue cell interactions in human colorectal adenocarcinoma. Transfer of platelet-derived growth factor-AB/BB to stromal cells.The American journal of pathology · 1997Article
- Chinese hamster ovary cells produce sufficient recombinant insulin-like growth factor I to support growth in serum-free medium. Serum-free growth of IGF-I-producing CHO cells.Cytotechnology · 1997Article
- Scatter factor binds to thrombospondin and other extracellular matrix components.The American journal of pathology · 1996Article
- Processing of mutated human proinsulin to mature insulin in the non-endocrine cell line, CHO.Cytotechnology · 1996Article
- Platelet-derived growth factor BB induces functional vascular anastomoses in vivo.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Platelet-derived growth factor ligand and receptor expression by large vessel endothelium in vivo.The American journal of pathology · 1995Article
- Specific inhibition of eIF-5A and collagen hydroxylation by a single agent. Antiproliferative and fibrosuppressive effects on smooth muscle cells from human coronary arteries.The Journal of clinical investigation · 1995Article
- Signals controlling the expression of PDGF.Molecular biology reportsReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
PDGF is a powerful mitogen initially identified within platelets, but also shown to be produced by a wide variety of cell types. PDGF is encoded on two separate genes. These give rise to three polypeptides, PDGF B and two forms of PDGF A (SA and LA), resulting from alternative splicing of the PDGF A gene primary transcript. We report that in CHO cells transfected with PDGF gene constructs and producing moderate levels of PDGF homodimers, much of the PDGF LA and B produced, but little if any SA, is found in the matrix laid down beneath the cells. Immunoreactive PDGF in cells, and in matrix below expressing cells, was visualized by laser confocal microscopy. Western blotting of protein in matrix extracts, cell extracts, and secreted into the growth medium was used to demonstrate that the range of PDGF A polypeptides seen in the matrix was overlapping with those reported previously to be cell associated in cell types such as NIH3T3 and COS 7. This suggests that attachment to matrix or cell surface may be alternative fates for these polypeptides, with fate dependent on the characteristics of the producing cells. Immunoreactive PDGF A and B could be partially released by incubation of matrix material with heparin but not with other glycosaminoglycans. Digestion of matrix with chondroitin ABC lyase but not heparitinase or collagenase displaced some PDGF from its attachment sites. The results indicate attachment of PDGF to matrix proteoglycans, at least partly through the glycosaminoglycan moieties, and perhaps to additional components. The significance of matrix deposition for PDGF action is discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.