ReviewExperientia1993
Isolation and purification of proteoglycans.
Review in Experientia, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
8 citing papers in PubMed, 28 citations in OpenAlex.
- Effects of hemarthrosis on cartilage and synovium in rabbits.European journal of trauma and emergency surgery : official publication of the European Trauma Society · 2017Article
- Age estimation based on aspartic acid racemization in human sclera.International journal of legal medicine · 2016Article
- Regional variation in human supraspinatus tendon proteoglycans: decorin, biglycan, and aggrecan.Connective tissue research · 2012Article
- Stability and function of glycosaminoglycans in porcine bioprosthetic heart valves.Biomaterials · 2006Article
- Proteoglycans: pericellular and cell surface multireceptors that integrate external stimuli in the mammary gland.Journal of mammary gland biology and neoplasia · 2001Review
- Purification and characterization of heparan sulphate proteoglycan from bovine brain.The Biochemical journal · 1999Article
- Preparation and isolation of neoglycoconjugates using biotin-streptavidin complexes.Glycoconjugate journal · 1999Article
- A step towards developing the expertise to control hunger and satiety: regulatory role of satiomem--a membrane proteoglycan.Neurochemical research · 1995Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Purification of a protein typically involves development of a quantitative assay to track protein integrity (e.g. enzyme activity) during subsequent isolation steps. The generalized procedure involves choosing the source of the protein, defining extraction conditions, developing bulk purification methods followed by refined, more selective methods. The purification of proteoglycans is often complicated by a) limited source quantities, b) necessity of chaotropic solvents for efficient extraction, c) their large molecular size and d) lack of defined functions to enable purity (i.e. activity, conformation) to be assessed. Because the usual goal of proteoglycan purification is physical characterization (intact molecular weight, core protein and glycosaminoglycan class and size), the problems of a suitable assay and/or native conformation are avoided. The 'assay' for tracking proteoglycan isolation typically utilizes uronic acid content or radiolabel incorporation as a marker. Once extracted from their cellular/extracellular environment, proteoglycans can be isolated by density gradient centrifugation and/or column chromatography techniques. Recent advances in the composition of chromatographic supports have enabled the application of ion-exchange, gel permeation, hydrophobic interaction and affinity chromatography resins using efficient high-pressure liquid chromatography to proteoglycan purification.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.