Evidence map›Paper›PMID 8499685›Full record

ArticleJournal of assisted reproduction and genetics1993

A fast and efficient method for simultaneous X and Y in situ hybridization of human blastomeres.

S Munné, H U Weier, J Stein, J Grifo, J Cohen

Abstract read
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In one paragraph

Article in Journal of assisted reproduction and genetics, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed, 1 pooled it
19.7field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 1 synthesis or guideline pooled it, 116 citations in OpenAlex.

  1. Pooled it
  2. Novel embryo selection strategies-finding the right balance.Frontiers in reproductive health · 2023
    Article
  3. Review
  4. Ethical considerations of gene editing and genetic selection.Journal of general and family medicine · 2020
    Article
  5. The state of "freeze-for-all" in human ARTs.Journal of assisted reproduction and genetics · 2016
    Review
  6. Review
  7. Advances in embryo selection methods.F1000 biology reports · 2012
    Article
  8. Establishment of a simple and useful way for preimplantation genetic diagnosis of chromosomal diseases.Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban · 2007
    Article
  9. Article
  10. Article
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  16. Fluorescent PCR: a new technique for PGD of sex and single-gene defects.Journal of assisted reproduction and genetics · 1996
    Review
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

S MunnéGamete and Embryo Research Laboratory, New York Hospital-Cornell University Medical Center, New York 10021.
H U Weier
J Stein
J Grifo
J Cohen
Cornell University · USNewYork–Presbyterian Hospital · USUniversity of California, San Francisco · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeTo use a 6-hr fluorescence in situ hybridization (FISH) procedure involving fluorochrome-labeled probes to determine the gender of blastomeres from arrested biopsied human embryos.

resultsSimultaneous detection of X and Y chromosomes was performed on 68 blastomeres with this technique. The FISH efficiency for gender determination was 95.5% (65/68). In addition, rehybridization with chromosome 8-specific probes was performed to determine the ploidy of blastomeres with more than two sex chromosomes.

conclusionThis technique offers an alternative to polymerase chain reaction for the preimplantation diagnosis of X-linked diseases and can also be used for ploidy assessment.

Indexed as

In Situ Hybridization, FluorescenceX ChromosomeY ChromosomeBiopsyBlastomeresChromosome AberrationsChromosome DisordersFixativesHumansPloidiesReproducibility of ResultsSex Determination AnalysisTime FactorsFixatives

Identifiers

PMID8499685
OpenAlexW2067124348

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.