ArticlePlant molecular biology1993
Construction of a Chlamydomonas reinhardtii mutant with an intronless psbA gene.
Article in Plant molecular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 75 citations in OpenAlex.
- Article
- An All-Green Photo-Electrochemical Biosensor Using Microalgae Immobilized on Eco-Designed Lignin-Based Screen-Printed Electrodes to Detect Sustainable Nanoherbicides.International journal of molecular sciences · 2023Article
- A dual electro-optical biosensor based on Chlamydomonas reinhardtii immobilised on paper-based nanomodified screen-printed electrodes for herbicide monitoring.Journal of nanobiotechnology · 2021Article
- Targeting mutations to the plastidial psbA gene of Chlamydomonas reinhardtii without direct positive selection.Scientific reports · 2019Article
- Dynamics Properties of Photosynthetic Microorganisms Probed by Incoherent Neutron Scattering.Biophysical journal · 2019Article
- Photosystem-II D1 protein mutants of Chlamydomonas reinhardtii in relation to metabolic rewiring and remodelling of H-bond network at QScientific reports · 2018Article
- A nucleus-encoded chloroplast protein regulated by iron availability governs expression of the photosystem I subunit PsaA in Chlamydomonas reinhardtii.Plant physiology · 2015Article
- A powerful molecular engineering tool provided efficient Chlamydomonas mutants as bio-sensing elements for herbicides detection.PloS one · 2013Article
- Article
- Article
- Structure-based design of novel Chlamydomonas reinhardtii D1-D2 photosynthetic proteins for herbicide monitoring.Protein science : a publication of the Protein Society · 2009Article
- Phylogenetic analyses suggest reverse splicing spread of group I introns in fungal ribosomal DNA.BMC evolutionary biology · 2005Article
- Minimal Extent of Sequence Homology Required for Homologous Recombination at the psbA Locus in Chlamydomonas reinhardtii Chloroplasts using PCR-generated DNA Fragments.Photosynthesis research · 2004Article
- Engineering the chloroplast encoded proteins of chlamydomonas.Photosynthesis research · 2004Article
- Mobile self-splicing group I introns from the psbA gene of Chlamydomonas reinhardtii: highly efficient homing of an exogenous intron containing its own promoter.Molecular and cellular biology · 2001Article
- Processing of a composite large subunit rRNA. Studies with chlamydomonas mutants deficient in maturation of the 23s-like rrna.The Plant cell · 1998Article
- Genetic engineering of thylakoid protein complexes by chloroplast transformation in Chlamydomonas reinhardtii.Photosynthesis research · 1995Article
- A robust protocol for site-directed mutagenesis of the D1 protein inChlamydomonas reinhardtii: A PCR-splicedpsbA gene in a plasmid conferring spectinomycin resistance was introduced into apsbA deletion strain.Photosynthesis research · 1994Article
- Correct in vivo RNA splicing of a mitochondrial intron in algal chloroplasts.Nucleic acids research · 1994Article
- Redesign of the Chlamydomonas reinhardtii QPhysiologia plantarumArticle
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Efficient chloroplast transformation systems now available allow the manipulation of the evolutionarily highly conserved psbA gene in the eucaryotic organism Chlamydomonas reinhardtii. Two copies of this gene in the inverted repeat region of the chloroplast genome contain four large group I introns. To analyse possible functions of these introns and to generate a mutant for simplified psbA gene manipulations, a psbA cDNA fragment was introduced into a psbA deletion mutant using the biolistic transformation method. A transformant with no introns in the psbA gene has been obtained and represents the first example of the removal of a complete set of introns from a chloroplast gene. The newly generated strain is photosynthetically competent and contains no detectable recipient genome copies. The loss of all four introns appears to be phenotypically silent.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.