ArticleMolecular and cellular biology1993
Disruption of largest subunit RNA polymerase II genes in Trypanosoma brucei.
Article in Molecular and cellular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Article
- RNA polymerase I transcribes procyclin genes and variant surface glycoprotein gene expression sites in Trypanosoma brucei.Eukaryotic cell · 2003Article
- Activity of a trypanosome metacyclic variant surface glycoprotein gene promoter is dependent upon life cycle stage and chromosomal context.Molecular and cellular biology · 1998Article
- Mechanisms of developmental regulation in Trypanosoma brucei: a polypyrimidine tract in the 3'-untranslated region of a surface protein mRNA affects RNA abundance and translation.Nucleic acids research · 1997Article
- The PARP promoter of Trypanosoma brucei is developmentally regulated in a chromosomal context.Nucleic acids research · 1996Article
- An efficient method for stable transfection of bloodstream-form Trypanosoma brucei.Nucleic acids research · 1996Article
- Control of gene expression in trypanosomes.Microbiological reviews · 1995Review
- PARP promoter-mediated activation of a VSG expression site promoter in insect form Trypanosoma brucei.Nucleic acids research · 1995Article
- A conserved upstream element is essential for transcription of the Leishmania tarentolae mini-exon gene.The EMBO journal · 1994Article
- Hierarchies of RNA-processing signals in a trypanosome surface antigen mRNA precursor.Molecular and cellular biology · 1994Article
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5 authors.
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Abstract
Two types of largest subunit RNA polymerase II (pol II) genes (pol IIA and pol IIB), differing in 3 amino acid substitutions, are encoded in the Trypanosoma brucei (stock 427-60) genome. As a result, the alpha-amanitin-resistant transcription of the procyclic acidic repetitive protein (PARP) and variant surface glycoprotein (VSG) genes was proposed to involve a modified, alpha-amanitin-resistant form of the largest subunit of pol II. Alternatively, pol I could transcribe the PARP and VSG genes. To discriminate between these two models, we deleted the N-terminal domain (about one-third of the polypeptide), which encodes the amino acid substitutions which discriminated the pol IIA and pol IIB genes, at both pol IIB alleles. The pol IIB- trypanosomes still transcribe the PARP genes and the VSG gene promoter region in insect-form trypanosomes by alpha-amanitin-resistant RNA polymerases, while control housekeeping genes are transcribed in an alpha-amanitin-sensitive manner, presumably by pol IIA. We conclude that the alpha-amanitin-resistant transcription of protein coding genes in T. brucei is not mediated by a diverged form of the largest subunit of pol II and that the presence of both the pol IIA and pol IIB genes is not essential for trypanosome viability. This conclusion was further supported by the finding that individual trypanosome variants exhibited allelic heterogeneity for the previously identified amino acid substitutions and that various permutations of the polymorphic amino acids generate at least four different types of largest subunit pol II genes. The expression of the PARP genes and the VSG gene promoter region by alpha-amanitin-resistant RNA polymerases in the pol IIB- trypanosomes provides evidence for transcription of these genes by pol I.
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