ArticleMolecular and cellular biology1993
Mouse heat shock transcription factors 1 and 2 prefer a trimeric binding site but interact differently with the HSP70 heat shock element.
Article in Molecular and cellular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 107 citations in OpenAlex.
- Role of HSF1 in cell division, tumorigenesis and therapy: a literature review.Cell division · 2025Review
- Screening and Structural Characterization of Heat Shock Response Elements (HSEs) inInternational journal of molecular sciences · 2024Article
- PARP1 Might Substitute HSF1 to Reactivate Latent HIV-1 by Binding to Heat Shock Element.Cells · 2022Article
- p53-mediated miR-18 repression activates HSF2 for IGF-IIR-dependent myocyte hypertrophy in hypertension-induced heart failure.Cell death & disease · 2017Article
- Metabolic control of the proteotoxic stress response: implications in diabetes mellitus and neurodegenerative disorders.Cellular and molecular life sciences : CMLS · 2016Review
- Expression of HSF2 decreases in mitosis to enable stress-inducible transcription and cell survival.The Journal of cell biology · 2014Article
- Heat shock transcription factor 1 is activated as a consequence of lymphocyte activation and regulates a major proteostasis network in T cells critical for cell division during stress.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- Transcriptional response to stress in the dynamic chromatin environment of cycling and mitotic cells.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Recent gene duplication and subfunctionalization produced a mitochondrial GrpE, the nucleotide exchange factor of the Hsp70 complex, specialized in thermotolerance to chronic heat stress in Arabidopsis.Plant physiology · 2012Article
- Inhibition of heat shock transcription factor binding by a linear polyamide binding in an unusual 1:1 mode.Chembiochem : a European journal of chemical biology · 2012Article
- Promotion of heat shock factor Hsf1 degradation via adaptor protein filamin A-interacting protein 1-like (FILIP-1L).The Journal of biological chemistry · 2011Article
- A novel transcriptional repressor, Rhit, is involved in heat-inducible and age-dependent expression of Mpv17-like protein, a participant in reactive oxygen species metabolism.Molecular and cellular biology · 2010Article
- Heterotrimerization of heat-shock factors 1 and 2 provides a transcriptional switch in response to distinct stimuli.Molecular biology of the cell · 2009Article
- Analysis of HSF4 binding regions reveals its necessity for gene regulation during development and heat shock response in mouse lenses.The Journal of biological chemistry · 2008Article
- Novel oxidative stress-responsive gene ERS25 functions as a regulator of the heat-shock and cell death response.The Journal of biological chemistry · 2008Article
- Inhibition of DNA binding by differential sumoylation of heat shock factors.Molecular and cellular biology · 2006Article
- Heat shock transcription factor 2 is not essential for embryonic development, fertility, or adult cognitive and psychomotor function in mice.Molecular and cellular biology · 2002Article
- Selective activation of the developmentally regulated Ha hsp17.6 G1 promoter by heat stress transcription factors.Plant physiology · 2002Article
- In vivo binding of active heat shock transcription factor 1 to human chromosome 9 heterochromatin during stress.The Journal of cell biology · 2002Article
- The loop domain of heat shock transcription factor 1 dictates DNA-binding specificity and responses to heat stress.Genes & development · 2001Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
To understand the function of multiple heat shock transcription factors in higher eukaryotes, we have characterized the interaction of recombinant mouse heat shock transcription factors 1 and 2 (mHSF1 and mHSF2) with their binding site, the heat shock element (HSE). For our analysis, we utilized the human HSP70 HSE, which consists of three perfect 5'-nGAAn-3' sites (1, 3, and 4) and two imperfect sites (2 and 5) arranged as tandem inverted repeats. Recombinant mHSF1 and mHSF2, which exist as trimers in solution, both bound specifically to this HSE and stimulated transcription of a human HSP70-CAT construct in vitro. Footprinting analyses revealed differential binding of mHSF1 and mHSF2 to the HSP70 HSE. Specifically, mHSF1 bound all five pentameric sites, whereas mHSF2 failed to interact with the first site of the HSE but bound to sites 2 to 5. Missing-nucleoside analysis demonstrated that the third and fourth nGAAn sites were essential for mHSF1 and mHSF2 binding. The binding of the initial mHSF1 trimer to the HSE exhibited preference for sites 3, 4, and 5, and then binding of a second trimer occurred at sites 1 and 2. These results suggest that HSF may recognize its binding site through the dyad symmetry of sites 3 and 4 but requires an adjacent site for stable interaction. Our data demonstrate that mHSF1 and mHSF2 bind specifically to the HSE through major groove interactions. Methidiumpropyl-EDTA footprinting revealed structural differences in the first and third repeats of the HSE, suggesting that the DNA is distorted in this region. The possibility that the HSE region is naturally distorted may assist in understanding how a trimer of HSF can bind to what is essentially an inverted repeat binding site.
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