ArticleThe Journal of experimental medicine1993
Reconstitution of the functional receptors for murine and human interleukin 5.
Article in The Journal of experimental medicine, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- IL-5 antagonism reverses priming and activation of eosinophils in severe eosinophilic asthma.Mucosal immunology · 2024Article
- Structural basis of interleukin-5 dimer recognition by its α receptor.Protein science : a publication of the Protein Society · 2012Article
- Interleukin-5 and IL-5 receptor in health and diseases.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2011Review
- The role of interchain heterodisulfide formation in activation of the human common beta and mouse betaIL-3 receptors.The Journal of biological chemistry · 2010Article
- Two modes of beta-receptor recognition are mediated by distinct epitopes on mouse and human interleukin-3.The Journal of biological chemistry · 2010Article
- Crystallization and preliminary X-ray diffraction analysis of the ternary human GM-CSF receptor complex.Acta crystallographica. Section F, Structural biology and crystallization communications · 2008Article
- Slow-dissociation effect of common signaling subunit beta c on IL5 and GM-CSF receptor assembly.Cytokine · 2008Article
- Dissection of the cytoplasmic domains of cytokine receptors involved in STAT and Ras dependent proliferation.Oncogene · 2002Article
- Article
- Expression and in vitro properties of guinea pig IL-5: comparison to human and murine orthologs.Mediators of inflammation · 2000Article
- Dimeric RFX proteins contribute to the activity and lineage specificity of the interleukin-5 receptor alpha promoter through activation and repression domains.Molecular and cellular biology · 1999Article
- Allergen-induced increases in IL-5 receptor alpha-subunit expression on bone marrow-derived CD34+ cells from asthmatic subjects. A novel marker of progenitor cell commitment towards eosinophilic differentiation.The Journal of clinical investigation · 1997Article
- A critical role of Lyn and Fyn for B cell responses to CD38 ligation and interleukin 5.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Cytoplasmic domains of the common beta-chain of the GM-CSF/IL-3/IL-5 receptors that are required for inducing differentiation or clonal suppression in myeloid leukaemic cell lines.The EMBO journal · 1997Article
- CD38 ligation induces tyrosine phosphorylation of Bruton tyrosine kinase and enhanced expression of interleukin 5-receptor alpha chain: synergistic effects with interleukin 5.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Mutagenesis in the C-terminal region of human interleukin 5 reveals a central patch for receptor alpha chain recognition.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Activating point mutations in the common beta subunit of the human GM-CSF, IL-3 and IL-5 receptors suggest the involvement of beta subunit dimerization and cell type-specific molecules in signalling.The EMBO journal · 1995Article
- Detailed analysis of the IL-5-IL-5R alpha interaction: characterization of crucial residues on the ligand and the receptor.The EMBO journal · 1995Article
- Identification of receptor-binding domains on human interleukin 5 and design of an interleukin 5-derived receptor antagonist.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Sequential mutations in the interleukin-3 (IL3)/granulocyte-macrophage colony-stimulating factor/IL5 receptor beta-subunit genes are necessary for the complete conversion to growth autonomy mediated by a truncated beta C subunit.Molecular and cellular biology · 1995Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The murine interleukin 5 receptor (mIL-5R) is composed of two distinct subunits, alpha and beta. The alpha subunit (mIL-5R alpha) specifically binds IL-5 with low affinity. The beta subunit (mIL-5R beta) does not bind IL-5 by itself, but forms the high-affinity receptor with mIL-5R alpha. mIL-5R beta has been revealed to be the mIL-3R-like protein, AIC2B which is shared with receptors for IL-3 and granulocyte/macrophage colony-stimulating factor. We demonstrated here the reconstitution of the functional receptors for murine and human IL-5 on the mouse IL-2-dependent cell line, CTLL-2. CTLL-2 was transfected with the cDNAs for mIL-5R alpha and/or AIC2B. Only CTLL-2 transfectant expressing both mIL-5R alpha and AIC2B expressed the high-affinity receptor and proliferated in response to murine IL-5. Then CTLL-2 was transfected with the cDNAs for hIL-5R alpha and/or KH97 (beta c), the human homologue of AIC2B. Though beta c did not contribute much to binding affinity of hIL-5R, only CTLL-2 transfectant expressing both hIL-5R alpha and beta c proliferated in response to human IL-5. These results showed that the beta subunit is indispensable in IL-5 signal transduction. We further investigated the function of IL-5-specific alpha subunit in transmitting IL-5 signals. Mutant mIL-5R alpha, which lacks its whole cytoplasmic domain, was transfected into mouse IL-3-dependent cell line, FDC-P1 expressing AIC2B intrinsically. The resulting transfectant did not respond to IL-5, though the transfectant expressed the high-affinity IL-5R, indicating that the cytoplasmic portion of the alpha subunit also has some important role in IL-5-mediated signal transduction.
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