ArticleMolecular & general genetics : MGG1993
Modular organization and development activity of an Arabidopsis thaliana EF-1 alpha gene promoter.
Article in Molecular & general genetics : MGG, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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35 citing papers in PubMed.
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- The Rapid Methylation of T-DNAs UponFrontiers in plant science · 2019Article
- A polymorphic (GA/CT)n- SSR influences promoter activity of Tryptophan decarboxylase gene in Catharanthus roseus L. Don.Scientific reports · 2016Article
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- Comparison of the MpEF1α and CaMV35 promoters for application in Marchantia polymorpha overexpression studies.Transgenic research · 2014Article
- Isolation and characterization of three cassava elongation factor 1 alpha (MeEF1A) promoters.PloS one · 2014Article
- Pineapple translation factor SUI1 and ribosomal protein L36 promoters drive constitutive transgene expression patterns in Arabidopsis thaliana.Plant molecular biology · 2013Article
- TFE2 and GATA3 enhance induction of POU4F3 and myosin VIIa positive cells in nonsensory cochlear epithelium by ATOH1.Developmental biology · 2012Article
- DNA free energy-based promoter prediction and comparative analysis of Arabidopsis and rice genomes.Plant physiology · 2011Article
- Distribution of short interstitial telomere motifs in two plant genomes: putative origin and function.BMC plant biology · 2010Article
- Functional analysis of 5' untranslated region of a TIR-NBS-encoding gene from triploid white poplar.Molecular genetics and genomics : MGG · 2009Article
- Promoter-proximal introns in Arabidopsis thaliana are enriched in dispersed signals that elevate gene expression.The Plant cell · 2008Article
- Saccharomyces cerevisiae FKBP12 binds Arabidopsis thaliana TOR and its expression in plants leads to rapamycin susceptibility.BMC plant biology · 2007Article
- Seed-specific expression of sesame microsomal oleic acid desaturase is controlled by combinatorial properties between negative cis-regulatory elements in the SeFAD2 promoter and enhancers in the 5'-UTR intron.Molecular genetics and genomics : MGG · 2006Article
- Effect of 5'UTR introns on gene expression in Arabidopsis thaliana.BMC genomics · 2006Article
- The cauliflower mosaic virus 35S promoter extends into the transcribed region.Journal of virology · 2004Article
- Methylmalonate-semialdehyde dehydrogenase is induced in auxin-stimulated and zinc-stimulated root formation in rice.Plant cell reports · 2004Article
- The Aa-Pri4 gene, specifically expressed during fruiting initiation in the Agrocybe aegerita complex, contains an unusual CT-rich leader intron within the 5' uncoding region.Current genetics · 2003Article
- Characterization of cis-regulatory regions responsible for developmental regulation of the gibberellin biosynthetic gene GA1 in Arabidopsis thaliana.Plant molecular biology · 2002Article
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6 authors.
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Abstract
The activity of the Arabidopsis thalana A1 EF-1 alpha gene promoter was analyzed in transgenic Arabidopsis plants. The 5' upstream sequence of the A1 gene and several promoter deletions were fused to the beta-glucuronidase (GUS) coding region. Promoter activity was monitored by quantitative and histochemical assays of GUS activity. The results show that the A1 promoter exhibits a modular organization. Sequences both upstream and downstream relative to the transcription initiation site are involved in quantitative and tissue-specific expression during vegetative growth. One upstream element may be involved in the activation of expression in meristematic tissues; the downstream region, corresponding to an intron within the 5' non-coding region (5'IVS), is important for expression in roots; both upstream and downstream sequences are required for expression in leaves, suggesting combinatorial properties of EF-1 alpha cis-regulatory elements. This notion of specific combinatorial regulation is reinforced by the results of transient expression experiments in transfected Arabidopsis protoplasts. The deletion of the 5'IVS has much more effect on expression when the promoter activity is under the control of A1 EF-1 alpha upstream sequences than when these upstream sequences were replaced by the 35S enhancer. Similarly, a synthetic oligonucleotide corresponding to an A1 EF-1 alpha upstream cis-acting element (the TEF1 box), is able to restore partially the original activity when fused to a TEF1-less EF1-alpha promoter but has no significant effect when fused to an enhancer-less 35S promoter.
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